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Development of indirect enzyme-linked immunosorbent assay for diagnosis of canine leptospirosis.

作者信息

Sathiyamoorthy A, Selvaraju G, Palanivel K M, Srinivasan And P

机构信息

Department of Veterinary Preventive Medicine, Veterinary College and Research Institute, Namakkal - 637 002, Tamil Nadu, India.

出版信息

Vet World. 2017 May;10(5):530-535. doi: 10.14202/vetworld.2017.530-535. Epub 2017 May 20.

DOI:10.14202/vetworld.2017.530-535
PMID:28620258
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5465768/
Abstract

AIM

This study was taken up to develop an indirect enzyme-linked immunosorbent assay (i-ELISA) for screening antibodies against spp. in canines.

MATERIALS AND METHODS

An i-ELISA was developed using outer membrane protein extracted from serovar used for coating the well with concentration of 0.5 µg/µl. A total of 250 serum samples from clinically affected and apparently healthy dogs were collected along with relevant epidemiological data at Teaching Veterinary Clinical Complex, Veterinary College and Research Institute, Namakkal, and subjected to i-ELISA.

RESULTS

Out of 250 sera samples, 140 (56.00%) were found to be positive by i-ELISA. All the sera samples were subjected to microagglutination test (MAT) with panel of 12 different serovars. A total of 71 (28.40%) sera samples were positivity to MAT excluding the sera samples positive to serovars and in vaccinated dogs. Sensitivity and specificity of i-ELISA were higher in compared with MAT was 91.54% and 58.10%, respectively.

CONCLUSION

An indirect ELISA developed for the detection of canine antileptospiral antibodies proved to be highly sensitive, rapid and easy to perform and overcome the drawbacks of MAT.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2941/5465768/0d4b7cf4e2db/VetWorld-10-530-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2941/5465768/30c80b9bb5bf/VetWorld-10-530-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2941/5465768/3d9c7beb4a9f/VetWorld-10-530-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2941/5465768/0d4b7cf4e2db/VetWorld-10-530-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2941/5465768/30c80b9bb5bf/VetWorld-10-530-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2941/5465768/3d9c7beb4a9f/VetWorld-10-530-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2941/5465768/0d4b7cf4e2db/VetWorld-10-530-g003.jpg

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Marked increase in leptospirosis infections in humans and dogs in the Netherlands, 2014.2014 年荷兰人类和犬类钩端螺旋体病感染明显增加。
Euro Surveill. 2016 Apr 28;21(17). doi: 10.2807/1560-7917.ES.2016.21.17.30211.
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Meta-Analyses of Factors Associated with Leptospirosis in Domestic Dogs.家犬钩端螺旋体病相关因素的Meta分析
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