Cedillo Luciano, Ocampo-Bárcenas Azucena, Maldonado Israel, Valdez-Morales Francisco J, Camargo Felipe, López-Bayghen Esther
Laboratorio de Fertilización In Vitro and Laboratorio de Investigación y Diagnóstico Molecular, Instituto de Infertilidad y Genética, Ingenes México, Carretera México-Toluca No. 5420, Piso 6, Ofna 602 Col. El Yaqui, Del. Cuajimalpa, 05320 Mexico City, Mexico.
Facultad de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, Mexico City, 04510 Mexico.
Fertil Res Pract. 2016 Nov 25;2:13. doi: 10.1186/s40738-016-0027-4. eCollection 2016.
Preimplantation genetic screening (PGS) is an important procedure for in vitro fertilization (IVF). A key step of PGS, blastomere removal, is abundant with many technical issues. The aim of this study was to compare a more simple procedure based on the Stipper Micropipetter, named S-biopsy, to the conventional aspiration method.
On Day 3, 368 high-quality embryos (>7 cells on Day3 with <10% fragmentation) were collected from 38 women. For each patient, their embryos were equally separated between the conventional method ( = 188) and S-biopsy method ( = 180). The conventional method was performed using a standardized protocol. For the S-biopsy method, a laser was used to remove a significantly smaller portion of the zona pellucida. Afterwards, the complete embryo was aspirated with a Stripper Micropipetter, forcing the removal of the blastomere. Selected blastomeres went to PGS using CGH microarrays. Embryo integrity and blastocyst formation were assessed on Day 5. Differences between groups were assessed by either the Mann-Whitney test or Fisher Exact test.
Both methods resulted in the removal of only one blastomere. The S-biopsy and the conventional method did not differ in terms of affecting embryo integrity (95.0% vs. 95.7%) or blastocyst formation (72.7% vs. 70.7%). PGS analysis indicated that aneuploidy rate were similar between the two methods (63.1% vs. 65.2%). However, the time required to perform the S-biopsy method (179.2 ± 17.5 s) was significantly shorter (5-fold) than the conventional method.
The S-biopsy method is comparable to the conventional method that is used to remove a blastomere for PGS, but requires less time. Furthermore, due to the simplicity of the S-biopsy technique, this method is more ideal for IVF laboratories.
植入前基因筛查（PGS）是体外受精（IVF）的一项重要程序。PGS的一个关键步骤，即卵裂球去除，存在许多技术问题。本研究的目的是将一种基于剥离式微量移液器的更简单程序（称为S活检）与传统的抽吸方法进行比较。
在第3天，从38名女性中收集了368个高质量胚胎（第3天有>7个细胞且碎片<10%）。对于每位患者，其胚胎在传统方法（=188）和S活检方法（=180）之间平均分配。传统方法采用标准化方案进行。对于S活检方法，使用激光去除显著更小部分的透明带。然后，用剥离式微量移液器抽吸整个胚胎，促使卵裂球被去除。选取的卵裂球使用比较基因组杂交（CGH）微阵列进行PGS检测。在第5天评估胚胎完整性和囊胚形成情况。通过曼-惠特尼检验或费舍尔精确检验评估组间差异。
两种方法都仅去除了一个卵裂球。S活检和传统方法在影响胚胎完整性（95.0%对95.7%）或囊胚形成（72.7%对70.7%）方面没有差异。PGS分析表明，两种方法的非整倍体率相似（63.1%对65.2%）。然而，执行S活检方法所需的时间（179.2±17.5秒）明显比传统方法短（5倍）。
S活检方法与用于PGS去除卵裂球的传统方法相当，但所需时间更少。此外，由于S活检技术的简单性，该方法对IVF实验室更理想。
