• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用于硬组织潜在再生的纳米金刚石复合聚（ε-己内酯）纤维基质的制备与优化

Fabrication and optimization of Nanodiamonds-composited poly(ε-caprolactone) fibrous matrices for potential regeneration of hard tissues.

作者信息

Ahn Guk Young, Ryu Tae-Kyung, Choi Yu Ri, Park Ju Ri, Lee Min Jeong, Choi Sung-Wook

机构信息

Department of Biotechnology, The Catholic University of Korea, 43 Jibong-ro, Wonmi-gu, Bucheon-si, Gyeonggi-do 420-743 Republic of Korea.

出版信息

Biomater Res. 2018 May 30;22:16. doi: 10.1186/s40824-018-0126-x. eCollection 2018.

DOI:10.1186/s40824-018-0126-x
PMID:29862039
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5975567/
Abstract

BACKGROUND

Electrospun fibrous matrices are of great importance for tissue engineering and drug delivery device. However, relatively low mechanical strength of the fibrous matrix is one of the major disadvantages. NDs with a positive charge were selected to enhance the mechanical property of a composited fibrous matrix by inducing the intermolecular interaction between NDs and polymer chain. We prepared ND-composited poly (ε-caprolactone) (PCL) fibrous matrices by electrospinning and evaluated their performance in terms of mechanical strength and cell behaviors.

METHODS

A predetermined amounts of NDs (0.5, 1, 2 and 3 wt%) were added into PCL solution in a mixture of chloroform and 2,2,2-trifluoroethanol (8:2). ND-composited PCL (ND/PCL) fibrous matrices were prepared by electrospinning method. The tensile properties of the ND/PCL fibrous matrices were analyzed by using a universal testing machine. Mouse calvaria-derived preosteoblast (MC3T3-E1) was used for cell proliferation, alkaline phosphatase (ALP) assay, and Alizarin Red S staining.

RESULTS

The diameters of the fibrous matrices were adjusted to approximately 1.8 μm by changing process variables. The intermolecular interaction between NDs and PCL polymers resulted in the increased tensile strength and the favorable interfacial adhesion in the ND/PCL fibrous matrices. The ND/PCL fibrous matrix with 1 wt% of ND had the highest tensile strength among the samples and also improved proliferation and differentiation of MC3T3-E1 cells.

CONCLUSIONS

Compared to the other samples, the ND/PCL fibrous matrix with 1 wt% of ND concentration exhibited superior performances for MC3T3 cells. The ND/PCL fibrous matrix can be potentially used for bone and dental tissue engineering.

摘要

背景

电纺纤维基质对于组织工程和药物递送装置非常重要。然而，纤维基质相对较低的机械强度是其主要缺点之一。选择带正电荷的纳米金刚石（NDs）通过诱导NDs与聚合物链之间的分子间相互作用来增强复合纤维基质的机械性能。我们通过静电纺丝制备了ND复合聚（ε-己内酯）（PCL）纤维基质，并从机械强度和细胞行为方面评估了它们的性能。

方法

将预定量的NDs（0.5、1、2和3 wt%）添加到氯仿和2,2,2-三氟乙醇（8:2）的混合物中的PCL溶液中。通过静电纺丝法制备ND复合PCL（ND/PCL）纤维基质。使用万能试验机分析ND/PCL纤维基质的拉伸性能。使用小鼠颅骨来源的前成骨细胞（MC3T3-E1）进行细胞增殖、碱性磷酸酶（ALP）测定和茜素红S染色。

结果

通过改变工艺变量将纤维基质的直径调整至约1.8μm。NDs与PCL聚合物之间的分子间相互作用导致ND/PCL纤维基质的拉伸强度增加和良好的界面粘附性。在样品中，含1 wt% ND的ND/PCL纤维基质具有最高的拉伸强度，并且还改善了MC3T3-E1细胞的增殖和分化。

结论

与其他样品相比，含1 wt% ND浓度的ND/PCL纤维基质对MC3T3细胞表现出优异的性能。ND/PCL纤维基质可潜在地用于骨和牙科组织工程。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e939/5975567/c84bb1005337/40824_2018_126_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e939/5975567/76836166a0de/40824_2018_126_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e939/5975567/fac039254ac4/40824_2018_126_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e939/5975567/9b2e6a39faed/40824_2018_126_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e939/5975567/71f6d213ce77/40824_2018_126_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e939/5975567/aa9f709acf59/40824_2018_126_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e939/5975567/c84bb1005337/40824_2018_126_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e939/5975567/76836166a0de/40824_2018_126_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e939/5975567/fac039254ac4/40824_2018_126_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e939/5975567/9b2e6a39faed/40824_2018_126_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e939/5975567/71f6d213ce77/40824_2018_126_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e939/5975567/aa9f709acf59/40824_2018_126_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e939/5975567/c84bb1005337/40824_2018_126_Fig6_HTML.jpg

相似文献

1
Fabrication and optimization of Nanodiamonds-composited poly(ε-caprolactone) fibrous matrices for potential regeneration of hard tissues.用于硬组织潜在再生的纳米金刚石复合聚（ε-己内酯）纤维基质的制备与优化
Biomater Res. 2018 May 30;22:16. doi: 10.1186/s40824-018-0126-x. eCollection 2018.
2
Synthesis and characterization of CaO-loaded electrospun matrices for bone tissue engineering.用于骨组织工程的负载氧化钙的电纺基质的合成与表征
Clin Oral Investig. 2016 Nov;20(8):1921-1933. doi: 10.1007/s00784-015-1671-5. Epub 2015 Nov 27.
3
Electrospun composite matrices of poly(ε-caprolactone)-montmorillonite made using tenside free Pickering emulsions.使用无表面活性剂的皮克林乳液制备的聚（ε-己内酯）-蒙脱石电纺复合基质。
Mater Sci Eng C Mater Biol Appl. 2016 Dec 1;69:685-91. doi: 10.1016/j.msec.2016.07.046. Epub 2016 Jul 22.
4
In-situ polymerized polypyrrole nanoparticles immobilized poly(ε-caprolactone) electrospun conductive scaffolds for bone tissue engineering.原位聚合聚吡咯纳米粒子固定化聚（ε-己内酯）电纺导电支架用于骨组织工程
Mater Sci Eng C Mater Biol Appl. 2020 Sep;114:111056. doi: 10.1016/j.msec.2020.111056. Epub 2020 May 6.
5
Nanodiamond/poly-ε-caprolactone nanofibrous scaffold for wound management.纳米金刚石/聚己内酯纳米纤维支架用于伤口管理。
Mater Sci Eng C Mater Biol Appl. 2019 Jul;100:378-387. doi: 10.1016/j.msec.2019.02.110. Epub 2019 Mar 2.
6
Nanofibrous Mineralized Electrospun Scaffold as a Substrate for Bone Tissue Regeneration.纳米纤维矿化电纺支架作为骨组织再生的基质
J Biomed Nanotechnol. 2016 Nov;12(11):2076-82. doi: 10.1166/jbn.2016.2306.
7
Enhanced osteogenic differentiation of alendronate-conjugated nanodiamonds for potential osteoporosis treatment.阿仑膦酸盐偶联纳米金刚石增强成骨分化用于潜在的骨质疏松症治疗
Biomater Res. 2021 Sep 23;25(1):28. doi: 10.1186/s40824-021-00231-9.
8
Facile Fabrication of Composite Electrospun Nanofibrous Matrices of Poly(ε-caprolactone)-Silica Based Pickering Emulsion.聚己内酯-硅基 Pickering 乳液复合电纺纳米纤维基质的简易制备。
Langmuir. 2017 Aug 15;33(32):8062-8069. doi: 10.1021/acs.langmuir.7b02119. Epub 2017 Aug 4.
9
Electrospun fibrous scaffold of hydroxyapatite/poly (ε-caprolactone) for bone regeneration.用于骨再生的羟基磷灰石/聚（ε-己内酯）电纺纤维支架。
J Mater Sci Mater Med. 2012 Feb;23(2):547-54. doi: 10.1007/s10856-011-4495-0. Epub 2011 Dec 6.
10
Fabrication, characterization and determination of biological activities of poly(ε-caprolactone)/chitosan-caffeic acid composite fibrous mat for wound dressing application.用于伤口敷料应用的聚（ε-己内酯）/壳聚糖-咖啡酸复合纤维垫的制备、表征及生物活性测定
Int J Biol Macromol. 2016 Dec;93(Pt B):1549-1558. doi: 10.1016/j.ijbiomac.2016.06.065. Epub 2016 Jun 21.

引用本文的文献

1
Nanodiamond: a multifaceted exploration of electrospun nanofibers for antibacterial and wound healing applications.纳米金刚石：用于抗菌和伤口愈合应用的电纺纳米纤维的多方面探索。
J Nanobiotechnology. 2025 Apr 9;23(1):285. doi: 10.1186/s12951-025-03351-9.
2
and evaluation of electrospun poly (ε-caprolactone)/collagen scaffolds and Wharton's jelly mesenchymal stromal cells (hWJ-MSCs) constructs as potential alternative for skin tissue engineering.以及对静电纺丝聚（ε-己内酯）/胶原蛋白支架和脐带华通氏胶间充质基质细胞（hWJ-MSCs）构建物作为皮肤组织工程潜在替代物的评估。
Regen Ther. 2023 May 30;24:11-24. doi: 10.1016/j.reth.2023.05.005. eCollection 2023 Dec.
3

本文引用的文献

1
Enhanced osteogenic commitment of murine mesenchymal stem cells on graphene oxide substrate.氧化石墨烯基质上小鼠间充质干细胞的成骨分化增强
Biomater Res. 2018 Jan 2;22:1. doi: 10.1186/s40824-017-0112-8. eCollection 2018.
2
Modification and optimization of electrospun gelatin sheets by electron beam irradiation for soft tissue engineering.通过电子束辐照对用于软组织工程的电纺明胶片进行改性和优化。
Biomater Res. 2017 Jul 11;21:14. doi: 10.1186/s40824-017-0100-z. eCollection 2017.
3
Lamination of microfibrous PLGA fabric by electrospinning a layer of collagen-hydroxyapatite composite nanofibers for bone tissue engineering.
A systematic study on the use of multifunctional nanodiamonds for neuritogenesis and super-resolution imaging.
关于多功能纳米金刚石用于神经突生成和超分辨率成像的系统研究。
Biomater Res. 2023 Apr 27;27(1):37. doi: 10.1186/s40824-023-00384-9.
4
Hard Dental Tissues Regeneration-Approaches and Challenges.硬组织牙再生——方法与挑战
Materials (Basel). 2021 May 14;14(10):2558. doi: 10.3390/ma14102558.
5
Electropsun Polycaprolactone Fibres in Bone Tissue Engineering: A Review.电纺聚己内酯纤维在骨组织工程中的应用：综述。
Mol Biotechnol. 2021 May;63(5):363-388. doi: 10.1007/s12033-021-00311-0. Epub 2021 Mar 10.
6
High Nanodiamond Content-PCL Composite for Tissue Engineering Scaffolds.用于组织工程支架的高纳米金刚石含量聚己内酯复合材料。
Nanomaterials (Basel). 2020 May 15;10(5):948. doi: 10.3390/nano10050948.
7
Accelerated Osteogenic Differentiation of MC3T3-E1 Cells by Lactoferrin-Conjugated Nanodiamonds through Enhanced Anti-Oxidant and Anti-Inflammatory Effects.乳铁蛋白偶联纳米金刚石通过增强抗氧化和抗炎作用促进MC3T3-E1细胞的成骨分化
Nanomaterials (Basel). 2019 Dec 24;10(1):50. doi: 10.3390/nano10010050.
通过静电纺丝一层胶原-羟基磷灰石复合纳米纤维对微纤维聚乳酸-羟基乙酸共聚物（PLGA）织物进行层压以用于骨组织工程。
Biomater Res. 2017 Jun 13;21:11. doi: 10.1186/s40824-017-0097-3. eCollection 2017.
4
Study of myoblast differentiation using multi-dimensional scaffolds consisting of nano and micropatterns.使用由纳米和微图案组成的多维支架对成肌细胞分化的研究。
Biomater Res. 2017 Jan 11;21:1. doi: 10.1186/s40824-016-0087-x. eCollection 2017.
5
MG-63 osteoblast-like cell proliferation on auxetic PLGA scaffold with mechanical stimulation for bone tissue regeneration.在具有机械刺激的负泊松比聚乳酸-羟基乙酸共聚物支架上MG-63成骨样细胞增殖用于骨组织再生
Biomater Res. 2016 Oct 31;20:33. doi: 10.1186/s40824-016-0080-4. eCollection 2016.
6
Bone-targeted delivery of nanodiamond-based drug carriers conjugated with alendronate for potential osteoporosis treatment.基于纳米金刚石的载药载体与阿仑膦酸钠偶联物的骨靶向递药用于潜在的骨质疏松治疗。
J Control Release. 2016 Jun 28;232:152-60. doi: 10.1016/j.jconrel.2016.04.025. Epub 2016 Apr 17.
7
Preparation of collagen/hydroxyapatite/alendronate hybrid hydrogels as potential scaffolds for bone regeneration.制备胶原/羟基磷灰石/阿仑膦酸盐杂化水凝胶作为潜在的骨再生支架。
Colloids Surf B Biointerfaces. 2016 Jul 1;143:81-87. doi: 10.1016/j.colsurfb.2016.03.025. Epub 2016 Mar 11.
8
Electrospun gelatin/poly(ε-caprolactone) fibrous scaffold modified with calcium phosphate for bone tissue engineering.用于骨组织工程的磷酸钙改性电纺明胶/聚（ε-己内酯）纤维支架
Mater Sci Eng C Mater Biol Appl. 2014 Nov;44:183-90. doi: 10.1016/j.msec.2014.08.017. Epub 2014 Aug 13.
9
Performance of a multilayered small-diameter vascular scaffold dual-loaded with VEGF and PDGF.多层小直径血管支架双重负载 VEGF 和 PDGF 的性能。
Biomaterials. 2013 Oct;34(30):7302-13. doi: 10.1016/j.biomaterials.2013.06.006. Epub 2013 Jul 2.
10
Preparation and characterization of mesoporous bioactive glass/polycaprolactone nanofibrous matrix for bone tissues engineering.介孔生物活性玻璃/聚己内酯纳米纤维基质的制备及表征用于骨组织工程。
J Mater Sci Mater Med. 2012 Nov;23(11):2619-30. doi: 10.1007/s10856-012-4734-z. Epub 2012 Aug 9.