Gabardo Michelle de P, Sato José Paulo H, Daniel Amanda Gabriele de S, Andrade Mariana R, Pereira Carlos Eduardo R, Rezende Talita P, Otoni Luísa V Arantes, Rezende Lucas Avelino, Guedes Roberto M C
Department of Veterinary Clinic and Surgery, Veterinary School, Universidade Federal de Minas Gerais, Brazil.
Department of Veterinary Clinic and Surgery, Veterinary School, Universidade Federal de Minas Gerais, Brazil.
Vet Microbiol. 2017 Jun;205:75-79. doi: 10.1016/j.vetmic.2017.05.009. Epub 2017 May 12.
The aim of this study was to evaluate the fecal-oral transmission of L. intracellularis between mice and pigs. The study was divided into two parts. The first part aimed to determine whether mice could be infected by feces from pigs that are experimentally infected with L. intracellularis. Thirty-four Swiss mice received L. intracellularis PCR-positive feces from experimentally infected pigs (M1) for four consecutive days. Twelve other mice received swine negative feces (M2). Pools of mice feces were collected on alternating days post-exposure (dpe). The second part of the study aimed to test whether pigs could be infected when exposed to L. intracellularis PCR-positive feces from experimentally infected mice. Twelve 5-week-old pigs received feed mixed with L. intracellularis PCR-positive mice feces (P1), while the other two pigs received PCR-negative mice feces (P2) for four consecutive days. In the first study, the amount of L. intracellularis provided to M1 boxes per day was between 10 and 10. Mice shed, an average of 10 bacterial units every collection day. Three mice from M1 were positive for L. intracellularis by immunohistochemistry (IHC) at the end of the study. In the second part of the study, pigs in P1 received an average of 10 bacterial units per day. Ten pigs were infected by L. intracellularis based on positive qPCR and/or immunohistochemistry and serology results. These pigs shed an average of 10L. intracellularis/g of feces. Mice and pigs experimentally infected with L. intracellularis can infect each other, therefore, rodents should be considered players in the epidemiology of this disease in pig farms.
本研究的目的是评估胞内劳森菌在小鼠和猪之间的粪-口传播。该研究分为两部分。第一部分旨在确定小鼠是否会被经实验感染胞内劳森菌的猪的粪便感染。34只瑞士小鼠连续4天接受经实验感染猪的胞内劳森菌PCR阳性粪便（M1）。另外12只小鼠接受猪的阴性粪便（M2）。在暴露后交替日（dpe）收集小鼠粪便样本。研究的第二部分旨在测试猪暴露于经实验感染小鼠的胞内劳森菌PCR阳性粪便时是否会被感染。12只5周龄的猪连续4天接受与胞内劳森菌PCR阳性小鼠粪便混合的饲料（P1），而另外2只猪接受PCR阴性小鼠粪便（P2）。在第一项研究中，每天提供给M1组小鼠的胞内劳森菌数量在10到10之间。小鼠在每个收集日平均排出10个细菌单位。在研究结束时，M1组中有3只小鼠通过免疫组织化学（IHC）检测胞内劳森菌呈阳性。在研究的第二部分中，P1组的猪每天平均接受10个细菌单位。根据qPCR阳性和/或免疫组织化学及血清学结果，10头猪被胞内劳森菌感染。这些猪平均每克粪便排出10个胞内劳森菌。经实验感染胞内劳森菌的小鼠和猪可以相互感染，因此，在养猪场的这种疾病流行病学中应将啮齿动物视为传播因素。
