Xu Tingting, Guo Yong, Zhang Yuanyuan, Cao Kai, Zhou Xinchen, Qian Mengqi, Han Xinyan
Hainan Institute, Zhejiang University, Yazhou Bay Science and Technology City, Sanya 572025, China.
Key Laboratory of Animal Nutrition and Feed Science in East China, Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
Biology (Basel). 2023 Jun 17;12(6):879. doi: 10.3390/biology12060879.
(1) Background: () is an obligate intracellular Gram-negative bacterium that causes porcine ileitis. Pigs infected with have severe ileal lesions and show symptoms of diarrhea, indigestion, and growth retardation. Previous studies found that probiotic ferment (FAM) improved the growth performance, gut barrier, and function in piglets. Therefore, we aimed to reveal the mechanism that FAM alleviates negative performance in -challenged piglets by characterizing the changes in intestinal integrity, function, and gut microbiota following FAM supplementation. (2) Methods: Twenty-four healthy piglets were randomly allotted to four treatments. Three groups were challenged with ; both FAM addition and vaccination were performed to explore their positive effects on -infected piglets. (3) Results: Piglets infected with showed lower growth performance and typical pathological symptoms. Moreover, microscopic images showed that observed intestinal morphological damage could be repaired by FAM and vaccine. To explore the digestion of nutrients in piglets, both digestive enzyme activity and ileal transporter expression were performed to reveal the promoting effect of additives. Reduction of colonization intervention by FAM could also ameliorate abnormal differentiation and function of intestinal epithelial cells and alleviate severe inflammatory responses in piglets. Regarding the gut microbiota, both the structure and function of the ileal and colonic microbiota were altered following FAM supplementation. (4) Conclusions: In conclusion, probiotic ferment can reduce the colonization of in the ileum, improve intestinal damage, barrier function and microbiota structure, and enhance digestive enzyme activity and nutrient transport proteins expression, thereby improving piglet growth performance, which has the effect of preventing ileitis in pigs.
(1) 背景：（某细菌名称未给出）是一种专性细胞内革兰氏阴性菌，可引起猪回肠炎。感染该菌的猪会出现严重的回肠病变，并表现出腹泻、消化不良和生长迟缓等症状。先前的研究发现，益生菌发酵物（FAM）可改善仔猪的生长性能、肠道屏障和功能。因此，我们旨在通过表征补充FAM后肠道完整性、功能和肠道微生物群的变化，揭示FAM减轻受该菌挑战仔猪负面表现的机制。(2) 方法：将24只健康仔猪随机分为四组。三组用该菌进行攻毒；同时进行FAM添加和疫苗接种，以探究它们对感染该菌仔猪的积极影响。(3) 结果：感染该菌的仔猪生长性能较低且出现典型病理症状。此外，显微图像显示，观察到的肠道形态损伤可通过FAM和疫苗修复。为探究仔猪营养物质的消化情况，检测了消化酶活性和回肠转运蛋白表达，以揭示添加剂的促进作用。FAM减少该菌定植干预还可改善肠道上皮细胞的异常分化和功能，减轻仔猪的严重炎症反应。关于肠道微生物群，补充FAM后回肠和结肠微生物群的结构和功能均发生了改变。(4) 结论：总之，益生菌发酵物可减少该菌在回肠的定植，改善肠道损伤、屏障功能和微生物群结构，增强消化酶活性和营养转运蛋白表达，从而提高仔猪生长性能，具有预防猪回肠炎的作用。
