Märki Iwan, Rebeaud Fabien
Abionic SA, Biopôle, sect. Esplanade SE-A, Route de la Corniche 3, CH-1066, Epalinges, Switzerland.
Curr Allergy Asthma Rep. 2017 Jul;17(7):50. doi: 10.1007/s11882-017-0717-y.
This review discusses the recent advances in the development of IgE antibody assays based on nanotechnologies. IgE blood testing is an important part of the diagnostic workup of IgE-mediated hypersentivity. We also address the challenges in moving from an academic proof-of-concept to a product routinely used by allergy experts.
Several nanotechnologies have been applied to the field of IgE testing: nanoparticles are used either as a support to capture analytes or as a detection tool to enhance the measurement signal. Nanofluidics allows to reduce assay time by enhancing molecular interaction. Nanotechnologies bring forth new methods for in vitro IgE testing. Substantial advantages such as lower sample volume, shorter assay time, simplified procedures, and lower analytic sensitivity, without affecting test precision and accuracy, can be achieved thanks to nanotechnologies.
本综述讨论了基于纳米技术的IgE抗体检测方法的最新进展。IgE血液检测是IgE介导的超敏反应诊断检查的重要组成部分。我们还探讨了从学术概念验证转向过敏专家常规使用产品所面临的挑战。
几种纳米技术已应用于IgE检测领域：纳米颗粒既用作捕获分析物的载体，也用作增强测量信号的检测工具。纳米流体学通过增强分子相互作用来减少检测时间。纳米技术为体外IgE检测带来了新方法。借助纳米技术，可以实现诸如更低样本量、更短检测时间、简化程序以及更低分析灵敏度等显著优势，同时不影响检测的精密度和准确性。
