Instituto Interuniversitario de Investigación de Reconocimiento Molecular Y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, Camino de Vera S/N, 46022, Valencia, Spain.
Departamento de Química, Universitat Politècnica de València, Valencia, Spain.
Mikrochim Acta. 2023 Apr 3;190(5):166. doi: 10.1007/s00604-023-05756-5.
A high percentage of the population suffers from multiple food allergies justifying the importance of reliable diagnostic methods. Single-analyte solutions based on the determination of specific immunoglobulins E (sIgE) are safe and fast but are generally time-consuming and expensive. Thus sustainable microanalytical methods that provide multianalyte profiling information are highly demanded. This work presents the in vitro biosensing of specific IgE levels based on a reversed-phase allergen array. The approach consists of optical biosensing supported by direct multiplex immunoassays and on-disc technology. It identifies 12 sIgE associated with food allergies in a single analysis with a low serum sample volume (25 µL). After processing captured images, specific signals for each target biomarker correlate to their concentration. The assay analytically performs well with 0.3 IU/mL and 0.41 IU/mL as the detection and quantification limits in serum, respectively. This novel method achieves excellent clinical specificity (100%) and high sensitivity (91.1%), considering the diagnosis obtained by clinical history and ImmunoCAP analysis. The results demonstrate that microanalytical systems based on allergen arrays can potentially diagnose multiple food allergies and are easily implemented in primary care laboratory settings.
高比例的人口患有多种食物过敏症，这证明了可靠的诊断方法的重要性。基于特异性免疫球蛋白 E（sIgE）测定的单分析物解决方案既安全又快速，但通常耗时且昂贵。因此，需要可持续的微分析方法来提供多分析物分析信息。本工作提出了基于反相过敏原阵列的特异性 IgE 水平的体外生物传感。该方法包括光学生物传感，由直接多重免疫测定和盘上技术支持。它可以在单次分析中使用 25µL 的低血清样本量识别与食物过敏相关的 12 种 sIgE。在处理捕获的图像后，每个靶标生物标志物的特异性信号与其浓度相关。该测定在血清中具有 0.3 IU/mL 和 0.41 IU/mL 的检测和定量限，分析性能良好。考虑到临床病史和 ImmunoCAP 分析获得的诊断，该新方法具有 100%的出色临床特异性和 91.1%的高灵敏度。结果表明，基于过敏原阵列的微分析系统有可能诊断多种食物过敏症，并且易于在基层医疗实验室环境中实施。
