Jung Seung-Ryoung, Fujimoto Bryant S, Chiu Daniel T
Department of Chemistry and Bioengineering, University of Washington, Seattle, WA 98195, United States.
Department of Chemistry and Bioengineering, University of Washington, Seattle, WA 98195, United States.
Curr Opin Chem Biol. 2017 Aug;39:64-73. doi: 10.1016/j.cbpa.2017.06.004. Epub 2017 Jun 15.
Quantitative microscopy is needed to understand reactions or phenomena carried out by biological molecules such as enzymes, receptors, and membrane-localized proteins. Counting the biomolecules of interest in single organelles or cellular compartments is critical in these approaches. In this brief perspective, we focus on the development of quantitative fluorescence microscopies that measure the precise copy numbers of proteins in cellular organelles or purified samples. We introduce recent improvements in quantitative microscopies to overcome undercounting or overcounting errors in certain conditions. We conclude by discussing biological applications.
为了理解诸如酶、受体和膜定位蛋白等生物分子所进行的反应或现象，需要进行定量显微镜观察。在这些方法中，对单个细胞器或细胞区室中感兴趣的生物分子进行计数至关重要。在这篇简短的综述中，我们聚焦于定量荧光显微镜技术的发展，该技术可测量细胞器或纯化样品中蛋白质的精确拷贝数。我们介绍了定量显微镜技术的最新改进，以克服某些条件下的计数不足或计数过多误差。我们通过讨论生物学应用来结束本文。
