姜黄素介导的新型光敏化处理，用于真菌孢子和细胞的失活。

A novel photosensitization treatment for the inactivation of fungal spores and cells mediated by curcumin.

机构信息

Queensland Alliance for Agricultural and Food Innovation (QAAFI), The University of Queensland, Australia; Department of Food and Nutrition Sciences, King Faisal University, Saudi Arabia.

Department of Agriculture and Fisheries Queensland, Australia.

出版信息

J Photochem Photobiol B. 2017 Aug;173:301-306. doi: 10.1016/j.jphotobiol.2017.06.009. Epub 2017 Jun 8.

DOI:10.1016/j.jphotobiol.2017.06.009

PMID:28623822

Abstract

The global concerns regarding the emergence of fungicide-resistant strains and the impact of the excessive use of fungicidal practises on our health, food, and environment have increased, leading to a demand for alternative clean green technologies as treatments. Photosensitization is a treatment that utilises a photosensitiser, light and oxygen to cause cell damage to microorganisms. The effect of photosensitization mediated by curcumin on Aspergillus niger, Aspergillus flavus, Penicillium griseofulvum, Penicillium chrysogenum, Fusarium oxysporum, Candida albicans and Zygosaccharomyces bailii was investigated using three methods. The viability of spores/cells suspended in aqueous buffer using different concentrations of curcumin solution (100-1000μM) and light dose (0, 24, 48, 72 and 96J/cm) were determined. Spraying curcumin solution on inoculated surfaces of agar plates followed by irradiation and soaking spores/cells in curcumin solution prior to irradiation was also investigated. In aqueous mixtures, photosensitised spores/cells of F. oxysporum and C. albicans were inhibited at all light doses and curcumin concentrations, while inactivation of A. niger, A. flavus P. griseofulvum, P. chrysogenum and Z. bailii were highly significant (P<0.001) reduced by 99%, 88.9%, 78%, 99.7% and 99.2% respectively. On the surface of agar plates, spores/cells exposed to a light dose of 360J/cm sprayed with curcumin at 800μM showed complete inhibition for A. niger, F. oxysporum, C. albicans and Z. bailii, while A. flavus P. griseofulvum, and P. chrysogenum reduced by 75%, 80.4% and 88.5% respectively. Soaking spores/cells with curcumin solution prior to irradiation did not have a significant effect on the percentage reduction. These observations suggest that a novel photosensitization mediated curcumin treatment is effective against fungal spores/cells and the variation of percentage reduction was dependent on curcumin concentration, light dosage and fungal species.

摘要

全球范围内对真菌抗药性菌株的出现以及过度使用杀真菌剂对健康、食品和环境的影响的担忧日益增加，这导致人们对替代清洁绿色技术的需求增加，将其作为治疗方法。光动力疗法是一种利用光敏剂、光和氧气来破坏微生物细胞的治疗方法。本研究采用三种方法，研究了姜黄素介导的光动力疗法对黑曲霉、黄曲霉、桔青霉、产黄青霉、尖孢镰刀菌、白色念珠菌和酿酒酵母的影响。用不同浓度的姜黄素溶液（100-1000μM）和不同光剂量（0、24、48、72 和 96J/cm）测定悬浮在水缓冲液中的孢子/细胞的活力。还研究了在琼脂平板接种表面喷洒姜黄素溶液后进行照射以及在照射前将孢子/细胞浸泡在姜黄素溶液中的方法。在水混合物中，所有光剂量和姜黄素浓度下，尖孢镰刀菌和白色念珠菌的光敏孢子/细胞均受到抑制，而黑曲霉、黄曲霉、桔青霉、产黄青霉和酿酒酵母的失活率则分别显著（P<0.001）降低了 99%、88.9%、78%、99.7%和 99.2%。在琼脂平板表面，用 800μM 姜黄素喷雾，在 360J/cm 的光剂量下暴露的孢子/细胞对黑曲霉、尖孢镰刀菌、白色念珠菌和酿酒酵母完全抑制，而黄曲霉、桔青霉和产黄青霉的失活率分别降低了 75%、80.4%和 88.5%。照射前用姜黄素溶液浸泡孢子/细胞对降低率没有显著影响。这些观察结果表明，新型姜黄素介导的光动力疗法对真菌孢子/细胞有效，降低率的变化取决于姜黄素浓度、光剂量和真菌种类。