Mora-Macías J, Pajares A, Miranda P, Domínguez J, Reina-Romo E
Department of Mining, Mechanical, Energy and Building Engineering, University of Huelva, 21071 Palos de la Frontera, Huelva, Spain.
Department of Mechanical, Energy and Materials Engineering, University of Extremadura, 06006 Badajoz, Spain.
J Mech Behav Biomed Mater. 2017 Oct;74:236-244. doi: 10.1016/j.jmbbm.2017.05.031. Epub 2017 Jun 1.
Nanoindentation has been used successfully in the determination of the mechanical properties of bone. Its application in fracture healing provides information on the evolution of material properties of the woven bone during regeneration process. However, this technique has not been applied in assessing the mechanical properties of woven bone during distraction osteogenesis. Therefore, the aim of this work is to evaluate the spatial and temporal variations of the elastic modulus of the woven bone generated during the bone transport process. Callus samples were harvested from intervened animals at different time points during the bone transport process (35, 50, 79, 98, 161 and 525 days after surgery) for nanoindentation measurements. Results clearly showed that the mean elastic modulus of the woven bone increased during the bone transport process reaching 77% of value for cortical bone after 525 days (from 7GPa 35 days after surgery to 14GPa 525 days after surgery approximately). Woven bone generated during bone transport seems to present similar evolution of elastic modulus with time as values reported for fracture healing. Furthermore, different spatial variations of elastic modulus within the callus were found for different stages of the process.
纳米压痕技术已成功用于测定骨的力学性能。其在骨折愈合中的应用提供了有关再生过程中编织骨材料性能演变的信息。然而，该技术尚未应用于评估牵张成骨过程中编织骨的力学性能。因此，本研究的目的是评估骨运输过程中产生的编织骨弹性模量的时空变化。在骨运输过程中的不同时间点（手术后35、50、79、98、161和525天）从干预动物中采集骨痂样本进行纳米压痕测量。结果清楚地表明，编织骨的平均弹性模量在骨运输过程中增加，在525天后达到皮质骨值的77%（从手术后35天的7GPa到手术后525天的约14GPa）。骨运输过程中产生的编织骨似乎与骨折愈合报道的值一样，呈现出弹性模量随时间的相似演变。此外，在该过程的不同阶段，发现骨痂内弹性模量存在不同的空间变化。
