Department of Stomatology, School of Dentistry, University of Sao Paulo, Sao Paulo, Brazil.
Oral Medicine & Pathology, Department of Stomatology II, UFI 11/25, University of the Basque Country (UPV/EHU), Leioa, Spain.
Arch Oral Biol. 2017 Oct;82:109-114. doi: 10.1016/j.archoralbio.2017.06.003. Epub 2017 Jun 6.
The aim of this study was to identify the possible association between TLR polymorphisms and an increased risk of developing head and neck cancer, including oral (OSCC) and laryngeal squamous cell carcinoma (LSCC), and oral potentially malignant disorders, such as oral lichenoid disease (OLD), including oral lichen planus (OLP) and oral lichenoid lesions (OLL).
This case-control study included 40 OSCC, 35 LSCC, 175 OLD (129 OLP and 46 OLL) patients and 89 healthy controls, all of them from the Basque Country, Spain. Genetic polymorphisms in TLR1, TLR2, TLR4, TLR6, TLR9, and TLR10 were genotyped by TaqMan assays or pyrosequencing.
The chi-square analysis showed that the variant A of the SNP TLR2-rs4696480 polymorphism significantly increased the risk of OSCC (p=0.03) and OLL (p=0.02).
The TLR2-rs4696480 polymorphism may be relevant to OSCC and OLL susceptibility in this population encouraging further studies on the TLR2 pathway and its possible association with this group of oral potentially malignant disorders and oral cancer. This may also prove the use of TLR polymorphisms as risk markers for oral and laryngeal cancer.
本研究旨在确定 TLR 多态性与头颈部癌症（包括口腔（OSCC）和喉鳞状细胞癌（LSCC））以及口腔潜在恶性疾病（如口腔扁平苔藓（OLD），包括口腔扁平苔藓（OLP）和口腔扁平苔藓样病变（OLL））风险增加之间的可能关联。
本病例对照研究纳入了来自西班牙巴斯克地区的 40 名 OSCC 患者、35 名 LSCC 患者、175 名 OLD 患者（129 名 OLP 和 46 名 OLL）和 89 名健康对照者。TLR1、TLR2、TLR4、TLR6、TLR9 和 TLR10 的基因多态性通过 TaqMan 分析或焦磷酸测序进行基因分型。
卡方分析显示，SNP TLR2-rs4696480 多态性的变体 A 显著增加了 OSCC（p=0.03）和 OLL（p=0.02）的风险。
TLR2-rs4696480 多态性可能与该人群中 OSCC 和 OLL 的易感性相关，这鼓励进一步研究 TLR2 途径及其与这群口腔潜在恶性疾病和口腔癌的可能关联。这也可能证明 TLR 多态性可用作口腔和喉癌的风险标志物。
