Centro de Investigaciones Biológicas del Noroeste, kilómetro 1, carretera a San Juan de la costa, La Paz, BCS CP. 23201, Mexico.
Departamento de Investigaciones Científicas y Tecnológicas de la Universidad de Sonora, Colosio s/n. Col. Centro, Hermosillo, Sonora CP. 83000, Mexico.
J Invertebr Pathol. 2017 Sep;148:118-123. doi: 10.1016/j.jip.2017.06.003. Epub 2017 Jun 15.
Survival of Litopenaeus vannamei was evaluated during a Vibrio parahaemolyticus infection. This bacteria has been causing significant economic losses in the shrimp industry due to the appearance of early mortality syndrome (EMS), also known as acute hepatopancreatic necrosis disease (AHPND). Control of bacteria in ponds is difficult to achieve with antibiotics due to environmental infections and antibiotic resistance. New methods have been proposed to control and prevent the impact of bacterial infections. The physiological response indicated by plasma biochemical parameters in shrimp can determine their health and stress status. Meanwhile, shrimp immunology is the key factor in establishing strategies to control diseases. Immunostimulants are the best alternative to antibiotics to prevent or minimize disease damage, and at the same time, these stimulants improve the immune system in shrimp. Four diets containing 1.5, 2, 2.5 and 3% of Dunaliella sp. with high β-carotene content were tested in the present study. After 20days of feeding, organisms were infected with V. parahaemolyticus. Protein, glucose, lactate, triglyceride and cholesterol levels, as well as activity of prophenoloxidase and phenoloxidase, were determined 48 h post-infection (hpi). Shrimp fed a diet with 3% Dunaliella sp. showed the highest survival. Glucose, cholesterol, and triglyceride levels, as well as prophenoloxidase and phenoloxidase activity, were not observed to be suitable indicators during this bacterial infection. The results indicated that the inclusion of Dunaliella sp. in diet increases survival in L. vannamei infected with V. parahaemolyticus.
在副溶血弧菌感染期间，评估了凡纳滨对虾的存活率。由于早期死亡综合征（EMS），也称为急性肝胰腺坏死病（AHPND）的出现，这种细菌已导致虾养殖业遭受重大经济损失。由于环境感染和抗生素耐药性，用抗生素控制池塘中的细菌是困难的。已经提出了新的方法来控制和预防细菌感染的影响。虾血浆生化参数所指示的生理反应可以确定其健康和应激状态。同时，虾免疫学是控制疾病的策略的关键因素。免疫刺激剂是抗生素的最佳替代品，可以预防或最小化疾病损害，同时这些刺激剂可以改善虾的免疫系统。本研究测试了含有 1.5％、2％、2.5％和 3％高β-胡萝卜素含量的杜氏盐藻的四种饲料。喂养 20 天后，用副溶血弧菌感染生物体。感染后 48 小时测定蛋白质、葡萄糖、乳酸盐、甘油三酯和胆固醇水平以及原酚氧化酶和酚氧化酶的活性。摄食 3％杜氏盐藻的虾表现出最高的存活率。在这种细菌感染期间，葡萄糖、胆固醇和甘油三酯水平以及原酚氧化酶和酚氧化酶的活性都不被认为是合适的指标。结果表明，在感染副溶血弧菌的凡纳滨对虾饲料中添加杜氏盐藻可以提高存活率。
