Centro de Investigación en Alimentos y Desarrollo A.C (CIAD), Carretera a La Victoria S/N, CP. 83304, Hermosillo, Sonora, Mexico.
Departamento de Investigaciones Científicas y Tecnológicas (DICTUS), Universidad de Sonora, Blvd. Luis Donaldo Colosio S/N, CP. 83000, Hermosillo, Sonora, Mexico.
Sci Rep. 2020 Jul 10;10(1):11428. doi: 10.1038/s41598-020-68222-9.
This work aimed to evaluate the link between the occurrence/abundance of Vibrio populations and bacterial composition in shrimp's intestine (Litopenaeus vannamei) during post-larval ontogenetic development and in its culture water, and the correlation of these with environmental parameters. The total and metabolically active populations of Vibrio in the digestive tract of shrimp during its post-larval development were analysed using quantitative PCR (qPCR) and reverse transcription qPCR targeting the 16S rRNA gene sequence. A lab-scale shrimp bioassay was performed for 80 days in a recirculating aquarium under strictly controlled conditions. The results indicate that the Vibrio population from shrimp's gut is associated with its developmental stage and the environment. Multivariate analyses revealed that the presence of Vibrio spp. drove the studied system, but their metabolically active performance was related to earlier developmental stages in an aqueous environment. Also, the samples taken from water of culture units to compare the influence of the aquatic environment on the intestinal microbial community during shrimp's ontogenetic development showed significant differences. Finally, our results revealed that Vibrio is an important member of shrimp's gut microbiota; however, its metabolic activity seems to be highly regulated, possibly by the host and by the rest of the microbiota.
本研究旨在评估在对虾（Litopenaeus vannamei）幼体发育后期及其养殖水中，弧菌种群的发生/丰度与肠道细菌组成之间的关系，以及这些关系与环境参数的相关性。采用定量 PCR（qPCR）和针对 16S rRNA 基因序列的反转录 qPCR 分析了对虾幼体发育过程中肠道中总弧菌和代谢活跃弧菌的数量。在严格控制条件下，采用实验室规模的循环水族箱进行了为期 80 天的对虾生物测定。结果表明，对虾肠道中的弧菌种群与其发育阶段和环境有关。多元分析表明，弧菌属的存在驱动了所研究的系统，但它们的代谢活性与早期的水生环境阶段有关。此外，从养殖单元的水样中采集样本，以比较水生环境对虾幼体发育过程中肠道微生物群落的影响，结果显示存在显著差异。最后，我们的研究结果表明，弧菌是对虾肠道微生物群的重要组成部分；然而，其代谢活性似乎受到宿主和其他微生物群的高度调控。
