双螺杆挤出机预处理后蒸汽爆破玉米芯残渣的酶解。

Zheng Jun, Choo Kim, Bradt Chris, Lehoux Rick, Rehmann Lars

Department of Chemical and Biochemical Engineering, University of Western Ontario, 1151 Richmond Street, London, ON N6A 3K7, Canada.

Lambton College, 1457 London Road, Sarnia, ON N7S 6K4, Canada.

Biotechnol Rep (Amst). 2014 Jun 26;3:99-107. doi: 10.1016/j.btre.2014.06.008. eCollection 2014 Sep.

A modified twin-screw extruder incorporated with a filtration device was used as a liquid/solid separator for xylose removal from steam exploded corncobs. A face centered central composite design was used to study the combined effects of various enzymatic hydrolysis process variables (enzyme loading, surfactant addition, and hydrolysis time) with two differently extruded corncobs (7% xylose removal, 80% xylose removal) on glucose conversion. The results showed that the extrusion process led to an increase in cellulose crystallinity, while structural changes could also be observed via SEM. A quadratic polynomial model was developed for predicting the glucose conversion and the fitted model provided an adequate approximation of the true response as verified by the analysis of variance (ANOVA).

一种配备过滤装置的改良双螺杆挤出机被用作从蒸汽爆破玉米芯中去除木糖的液/固分离器。采用面心中央复合设计，研究了各种酶水解工艺变量（酶负载量、表面活性剂添加量和水解时间）与两种不同挤压玉米芯（木糖去除率7%、木糖去除率80%）对葡萄糖转化率的综合影响。结果表明，挤压过程导致纤维素结晶度增加，同时通过扫描电子显微镜也可观察到结构变化。建立了一个二次多项式模型来预测葡萄糖转化率，经方差分析（ANOVA）验证，拟合模型对真实响应提供了充分的近似。