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在添加木质素的基本培养基中通过高通量浊度测量筛选菌株。

Screening of strains in lignin supplemented minimal medium with high throughput turbidity measurements.

作者信息

Glaser Robert, Venus Joachim

机构信息

Leibniz-Institute of Agricultural Engineering Potsdam, Max-Eyth-Allee 100, Potsdam 14469, Germany.

出版信息

Biotechnol Rep (Amst). 2014 Aug 15;4:60-65. doi: 10.1016/j.btre.2014.08.001. eCollection 2014 Dec.

Abstract

The aim of this study was to extend the options for screening and characterization of microorganism through kinetic growth parameters. In order to obtain data, automated turbidimetric measurements were accomplished to observe the response of strains of . For the characterization, it was decided to examine the influence of varying concentrations of lignin with respect to bacterial growth. Different mathematical models are used for comparison: logistic, Gompertz, Baranyi and Richards and Stannard. The growth response was characterized by parameters like maximum growth rate, maximum population, and the lag time. In this short analysis we present a mathematical approach towards a comparison of different microorganisms. Furthermore, it can be demonstrated that lignin in low concentrations can have a positive influence on the growth of .

摘要

本研究的目的是通过动力学生长参数扩展微生物筛选和表征的选项。为了获取数据,进行了自动比浊测量以观察菌株的反应。为了进行表征,决定研究不同浓度木质素对细菌生长的影响。使用不同的数学模型进行比较:逻辑斯蒂模型、冈珀茨模型、巴拉尼模型、理查兹模型和斯坦纳德模型。生长反应通过最大生长速率、最大种群数量和延迟期等参数来表征。在这个简短的分析中,我们提出了一种比较不同微生物的数学方法。此外,可以证明低浓度的木质素对……的生长有积极影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3408/5466133/0da7c3762c14/gr1.jpg

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