U.S. Department of Agriculture, Renewable Product Technology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, 1815 N. University St., Peoria, IL 61604, USA.
Biotechnol Lett. 2010 Jun;32(6):823-8. doi: 10.1007/s10529-010-0222-z. Epub 2010 Feb 14.
A strain of Bacillus coagulans that converted mixed sugars of glucose, xylose, and arabinose to L: -lactic acid with 85% yield at 50 degrees C was isolated from composted dairy manure. The strain was tolerant to aldehyde growth inhibitors at 2.5 g furfural/l, 2.5 g 5-hydroxymethylfurfural/l, 2.5 g vanillin/l, and 1.2 g p-hydroxybenzaldehyde/l. In a simultaneous saccharification and fermentation process, the strain converted a dilute-acid hydrolysate of 100 g corn fiber/l to 39 g lactic acid/l in 72 h at 50 degrees C. Because of its inhibitor tolerance and ability to fully utilize pentose sugars, this strain has potential to be developed as a biocatalyst for the conversion of agricultural residues into valuable chemicals.
从堆肥的奶牛粪便中分离到一株凝结芽孢杆菌，它能在 50°C 下将葡萄糖、木糖和阿拉伯糖的混合糖转化为 L: -乳酸，产率为 85%。该菌株能耐受 2.5 g 糠醛/L、2.5 g 5-羟甲基糠醛/L、2.5 g 香草醛/L 和 1.2 g 对羟基苯甲醛/L 等醛类生长抑制剂。在同步糖化发酵过程中，该菌株能在 50°C 下将 100 g 玉米纤维/L 的稀酸水解液在 72 h 内转化为 39 g 乳酸/L。由于其抑制剂耐受性和能够完全利用戊糖，该菌株有可能被开发为一种生物催化剂，用于将农业废弃物转化为有价值的化学品。
