Sajjadi Seyed Ebrahim, Ghanadian Mustafa, Haghighi Mehrangiz
Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
Adv Biomed Res. 2017 Jun 6;6:66. doi: 10.4103/2277-9175.190980. eCollection 2017.
Little information is available about chemical components of the genus. A primary cytotoxicity screening on showed moderate cytotoxic activity against OVCAR-3 ovarian and HT-29 colon cancer cells. Therefore, the aim of this study is a phytochemical investigation to identify the compounds responsible for this bioactivity.
Extraction was done through percolation and fractionations by reverse phase column chromatography and normal column chromatography. Using standard 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay Fr.b8 with moderate cytotoxicity was selected for identification of major components. Fr.b8 was subjected to polyamide column chromatography. More purification was done using a new modified recycle high-performance liquid chromatography (HPLC) with flow splitter.
Two known compounds: Apigenin (flavone) and caffeic acid (phenolic acid) were obtained from phenolic bioactive fraction for the first time from this plant.
Apigenin and caffeic acid with known antitumor and matrix metalloproteinase inhibitory effects seem to be the bioactive components responsible for moderate cytotoxicity of phenolic fraction. Recycle HPLC following with flow splitting is a new method useful for isolation of closely eluted compounds in HPLC chromatogram.
关于该属的化学成分信息较少。对[未提及的对象]进行的初步细胞毒性筛选显示，其对OVCAR - 3卵巢癌细胞和HT - 29结肠癌细胞具有中等细胞毒性活性。因此，本研究的目的是进行植物化学研究，以鉴定具有这种生物活性的化合物。
通过渗漉法进行提取，并采用反相柱色谱和正相柱色谱进行分离。使用标准的3 -（4,5 - 二甲基噻唑 - 2 - 基）- 2,5 - 二苯基四氮唑溴盐法，选择具有中等细胞毒性的Fr.b8进行主要成分鉴定。将Fr.b8进行聚酰胺柱色谱分离。使用带有分流器的新型改良循环高效液相色谱（HPLC）进行进一步纯化。
从该植物的酚类生物活性组分中首次获得了两种已知化合物：芹菜素（黄酮）和咖啡酸（酚酸）。
具有已知抗肿瘤和基质金属蛋白酶抑制作用的芹菜素和咖啡酸似乎是酚类组分具有中等细胞毒性的生物活性成分。带有分流的循环HPLC是一种用于分离HPLC色谱图中紧密洗脱化合物的新方法。
