Phytochemistry Research Center and School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Islamic Republic of Iran.
Department of Pharmacognosy, Isfahan Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Islamic Republic of Iran.
Fitoterapia. 2013 Dec;91:87-94. doi: 10.1016/j.fitote.2013.08.016. Epub 2013 Sep 17.
Dichloromethane-acetone extract of Euphorbia erythradenia Bioss., a spurge endemic to the Iran, afforded four novel tetrahydroingenol diterpenes, one new myrsinane type diterpene, and two known triterpenes. Tetrahydroingenoids are novel compounds not only for the double bond reduction but also for their unique hydroxylation pattern at C-11 and C-13. The structures of these compounds were elucidated by spectroscopic methods, and especially 2D NMR measurements. The biological effects of the compounds were done by the MTT and Annexin V-FITC & PI staining assays on different cancer cell lines. The results obtained on EJ-138, CAOV-4, and OVCAR-3 cell lines suggested that tetrahydroingenanes inhibit cell proliferation through apoptosis in cancer cell lines. In conclusion, the new pattern of hydrogenation and hydroxylation of these compounds compared to other ingenoids, along with their apoptosis inducing properties on cancer cells, makes them of great interest for more investigation.
来自伊朗特有大戟属植物红大戟的二氯甲烷-丙酮提取物，提供了四个新型四氢异土木香二萜，一个新的半日花烷型二萜和两个已知的三萜。四氢异土木香二萜不仅具有双键还原，而且还具有 C-11 和 C-13 独特的羟基化模式，是新型化合物。通过光谱方法，特别是二维 NMR 测量，阐明了这些化合物的结构。通过 MTT 和 Annexin V-FITC 和 PI 染色测定法，在不同的癌细胞系上测试了化合物的生物学效应。在 EJ-138、CAOV-4 和 OVCAR-3 细胞系上获得的结果表明，四氢异土木香烷通过细胞凋亡抑制癌细胞增殖。总之，与其他异土木香二萜相比，这些化合物的氢化和羟基化模式以及它们对癌细胞的诱导凋亡特性，使它们具有很大的研究价值。
