埃及患者白细胞介素-1A基因插入/缺失多态性及可能的高危因素与非酒精性脂肪性肝病的关联

Association of interleukin-1A insertion/deletion gene polymorphism and possible high risk factors with non-alcoholic fatty liver disease in Egyptian patients.

作者信息

Abdel-Hamed Asmaa R, Ghattas Maivel H, Mesbah Noha M, Saleh Samy M, Abo-Elmatty Dina M

机构信息

a Department of Biochemistry, Faculty of Pharmacy , Suez Canal University , Ismailia , Egypt.

b Department of Medical Biochemistry, Faculty of Medicine , Port Said University , Port Said , Egypt.

出版信息

Arch Physiol Biochem. 2017 Dec;123(5):330-333. doi: 10.1080/13813455.2017.1339717. Epub 2017 Jun 18.

DOI:10.1080/13813455.2017.1339717

PMID:28627263

Abstract

CONTEXT

Interleukin-1A (IL-1A) is a cytokine involved in inflammatory process. IL-1A (rs3783553) gene polymorphism is comprised in the regulation of IL-1A expression.

OBJECTIVE

This study aims to evaluate association of IL-1A (I/D) gene polymorphism with NAFLD and its component traits among Egyptian populations.

METHODS

The study included 75 healthy subjects and 75 patients with NAFLD. Different genotypes of IL-1A (I/D) gene polymorphism were determined by PCR-PAGE technique, serum IL-1A level and other biochemical parameters were measured.

RESULTS

The major D allele was significantly associated with NAFLD patients (p = .002). DD genotype showed a significant increase in BMI and decrease in HDL-C. Also serum IL-1A was significantly correlated with the DD genotype. Serum IL-1A showed a significant positive correlation with BMI, triglycerides, total cholesterol, LDL-C, VLDL-C and FBG, and a significant negative correlation with HDL-C.

CONCLUSIONS

Major D allele of IL-1A (I/D) gene polymorphism is associated with NAFLD in the Egyptian population.

摘要

背景

白细胞介素-1A（IL-1A）是一种参与炎症过程的细胞因子。IL-1A（rs3783553）基因多态性参与IL-1A表达的调控。

目的

本研究旨在评估埃及人群中IL-1A（I/D）基因多态性与非酒精性脂肪性肝病（NAFLD）及其组成特征之间的关联。

方法

该研究纳入了75名健康受试者和75名NAFLD患者。采用聚合酶链反应-聚丙烯酰胺凝胶电泳（PCR-PAGE）技术测定IL-1A（I/D）基因多态性的不同基因型，检测血清IL-1A水平及其他生化参数。

结果

主要的D等位基因与NAFLD患者显著相关（p = 0.002）。DD基因型的体重指数（BMI）显著升高，高密度脂蛋白胆固醇（HDL-C）降低。血清IL-1A也与DD基因型显著相关。血清IL-1A与BMI、甘油三酯、总胆固醇、低密度脂蛋白胆固醇（LDL-C）、极低密度脂蛋白胆固醇（VLDL-C）和空腹血糖（FBG）呈显著正相关，与HDL-C呈显著负相关。