Lian Yu-Ling, Cheng Ming-Jun, Zhang Xian-Xia, Wang Li
Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, P.R. China.
Mol Med Rep. 2017 Aug;16(2):1920-1926. doi: 10.3892/mmr.2017.6783. Epub 2017 Jun 15.
Pain is the most pronounced complaint of women with endometriosis, however the underlying mechanism is still poorly understood. In the present study, the authors evaluate the effect of transient receptor potential vanilloid type 1 (TRPV1) of dorsal root ganglia (DRG) on endometriosis-associated pain. A total of 36 SD rats were randomly divided into a sham group (n=9) and a Model group (n=27), accepted auto‑transplanted pieces of fat or uterus to the pelvic cavity. At 4 weeks, the Model group was randomly subdivided into the following groups: ENDO group (no treatment, n=9), BCTC group (Model + BCTC, an antagonist of TRPV1, n=9), Vehicle group (Model + cyclodextrin, the vehicle of BCTC, n=9). Tail‑flick test was performed prior to surgery, 1 h prior to and following treatment of BCTC or cyclodextrin. The expression of TRPV1, substance P (SP), calcitonin gene‑related peptide (CGRP) in L1‑L6 DRG was measured via immunohistochemistry, western blotting and RT‑qPCR. The results indicated that the Model group exhibited a significant decrease in tail flick latency compared to pre‑surgical baseline, and the expression of TRPV1, SP, CGRP protein and mRNA in L1‑L6 DRG significantly increased compared to the sham group. BCTC significantly improved tail flick latency, and downregulated the expression of TRPV1, SP and CGRP protein and mRNA levels in L1‑L6 DRG compared to ENDO group. However, there were no significant differences of those in Vehicle group compared with the ENDO group. Taken together, the current study provides evidence that TRPV1 expressed in DRG may serve an important role in endometriosis-associated pain.
疼痛是子宫内膜异位症女性最明显的症状，但其潜在机制仍知之甚少。在本研究中，作者评估背根神经节（DRG）中的瞬时受体电位香草酸受体1型（TRPV1）对子宫内膜异位症相关疼痛的影响。将36只SD大鼠随机分为假手术组（n = 9）和模型组（n = 27），分别将自体脂肪或子宫组织移植到盆腔。4周时，将模型组随机再分为以下几组：子宫内膜异位症组（不治疗，n = 9）、BCTC组（模型 + BCTC，TRPV1拮抗剂，n = 9）、溶媒组（模型 + 环糊精，BCTC的溶媒，n = 9）。在手术前、BCTC或环糊精治疗前1小时和治疗后进行甩尾试验。通过免疫组织化学、蛋白质印迹法和RT-qPCR检测L1-L6背根神经节中TRPV1、P物质（SP）、降钙素基因相关肽（CGRP）的表达。结果表明，与术前基线相比，模型组甩尾潜伏期显著缩短，与假手术组相比，L1-L6背根神经节中TRPV1、SP、CGRP蛋白和mRNA表达显著增加。与子宫内膜异位症组相比，BCTC显著改善了甩尾潜伏期，并下调了L1-L6背根神经节中TRPV1、SP和CGRP蛋白及mRNA水平的表达。然而，溶媒组与子宫内膜异位症组相比，上述指标无显著差异。综上所述，本研究提供了证据表明背根神经节中表达的TRPV1可能在子宫内膜异位症相关疼痛中起重要作用。
