Busato Alice, Bonafede Roberta, Bontempi Pietro, Scambi Ilaria, Schiaffino Lorenzo, Benati Donatella, Malatesta Manuela, Sbarbati Andrea, Marzola Pasquina, Mariotti Raffaella
Department of Computer Science, University of Verona, Verona, Italy.
Department of Neurological, Biomedical and Movement Sciences, University of Verona, Verona, Italy.
Curr Protoc Cell Biol. 2017 Jun 19;75:3.44.1-3.44.15. doi: 10.1002/cpcb.23.
Adipose stem cells (ASC) represent a promising therapeutic approach for neurodegenerative diseases. Most biological effects of ASC are probably mediated by extracellular vesicles, such as exosomes, which influence the surrounding cells. Current development of exosome therapies requires efficient and noninvasive methods to localize, monitor, and track the exosomes. Among imaging methods used for this purpose, magnetic resonance imaging (MRI) has advantages: high spatial resolution, rapid in vivo acquisition, and radiation-free operation. To be detectable with MRI, exosomes must be labeled with MR contrast agents, such as ultra-small superparamagnetic iron oxide nanoparticles (USPIO). Here, we set up an innovative approach for exosome labeling that preserves their morphology and physiological characteristics. We show that by labeling ASC with USPIO before extraction of nanovesicles, the isolated exosomes retain nanoparticles and can be visualized by MRI. The current work aims at validating this novel USPIO-based exosome labeling method by monitoring the efficiency of the labeling with MRI both in ASC and in exosomes. © 2017 by John Wiley & Sons, Inc.
脂肪干细胞（ASC）是治疗神经退行性疾病的一种很有前景的方法。ASC的大多数生物学效应可能是由细胞外囊泡介导的，比如外泌体，它们会影响周围的细胞。目前外泌体疗法的发展需要高效且无创的方法来定位、监测和追踪外泌体。在用于此目的的成像方法中，磁共振成像（MRI）具有优势：空间分辨率高、能在体内快速采集图像且无辐射。为了能用MRI检测到，外泌体必须用磁共振造影剂进行标记，比如超小超顺磁性氧化铁纳米颗粒（USPIO）。在此，我们建立了一种创新的外泌体标记方法，该方法能保留外泌体的形态和生理特性。我们表明，在提取纳米囊泡之前用USPIO标记ASC，分离出的外泌体就能保留纳米颗粒，并且可以通过MRI可视化。当前的工作旨在通过用MRI监测ASC和外泌体中标记的效率来验证这种基于USPIO的新型外泌体标记方法。© 2017约翰威立国际出版公司
