Chandrashekar R, Rao U R, Subrahmanyam D, Hopper K, Nelson D S, King M
Immunology. 1984 Nov;53(3):411-7.
The immune mechanisms operating on the sheath and the cuticle of the Brugia pahangi microfilariae have been studied in vitro. The intact and exsheathed parasites were used for this purpose with serum from albino rats immunized with sonicated homogenates of the parasites as the source of antibodies. The IgG component of the serum was found to promote adherence of rat leucocytes and death of the sheathed and exsheathed parasites in presence of fresh normal serum. EDTA and EGTA abolished the adherence activity suggesting the involvement of complement components via the classical pathway. Both macrophages and neutrophils participate in this reaction. Eosinophils exhibit marginal activity only on exsheathed parasites. The intensity of adherence and killing by neutrophils in the presence of immune serum was greater with the exsheathed microfilariae than with the sheathed ones.
已在体外研究了作用于彭亨布鲁线虫微丝蚴鞘膜和角质层的免疫机制。为此，使用完整的和脱鞘的寄生虫，以用寄生虫超声破碎匀浆免疫的白化大鼠血清作为抗体来源。发现血清中的IgG成分在新鲜正常血清存在的情况下可促进大鼠白细胞的黏附以及鞘膜和脱鞘寄生虫的死亡。EDTA和EGTA消除了黏附活性，表明补体成分通过经典途径参与其中。巨噬细胞和中性粒细胞均参与此反应。嗜酸性粒细胞仅对脱鞘寄生虫表现出轻微活性。在免疫血清存在的情况下，中性粒细胞对脱鞘微丝蚴的黏附及杀伤强度比对鞘膜微丝蚴的更大。
