Rao U R, Vickery A C, Nayar J K, Kwa B H
College of Public Health, Department of Environmental and Occupational Health, University of South Florida, Tampa 33612.
Gaoxiong Yi Xue Ke Xue Za Zhi. 1991 Apr;7(4):160-7.
The complement of fresh normal heterozygotic BALB/C (nu/+) and nude (nu/nu) mice was activated by sheathed microfilariae (Mf) of Brugia malayi and B. pahangi but not by B. patei. Mouse C3 was detected by immunofluorescence on the sheathed surface of B. malayi and B. pahangi Mf but not on B. patei Mf. The bound complement promoted macrophage and neutrophil mediated adherence and cytotoxicity to these Mf. However, BALB/C nu/+ or nu/nu mouse macrophages and neutrophils adhered to exsheathed Mf of B. patei and killed them in the presence of fresh normal mouse serum (NMS). C3 molecules were detected on the cuticle of exsheathed Mf of B. patei and B. malayi. Fresh NMS (nu/+ or nu/nu) depleted of complement factors by heating or by zymosan or anti-mouse C3, failed to promote cell adherence to these parasites. EDTA but not EGTA abolished the adherence activity to sheathed and exsheathed Mf of B. malayi, whereas both EGTA and EDTA abolished the adherence activity to exsheathed Mf of B. patei and sheathed Mf of B. pahangi suggesting the different pathways of complement activation by these closely related species. Macrophages and neutrophils from peritoneal exudate cells of nu/+ or nu/nu were equally potent in adherence and killing of Mf. Transfused B. patei but not B. malayi or B. pahangi Mf were recovered from the peritoneal cavity of BALB/C mice with no cell adherence and cytotoxicity.
新鲜正常的杂合BALB/C（nu/+）和裸鼠（nu/nu）的补体被马来布鲁线虫和彭亨布鲁线虫的带鞘微丝蚴（Mf）激活，但未被派氏布鲁线虫激活。通过免疫荧光在马来布鲁线虫和彭亨布鲁线虫Mf的带鞘表面检测到小鼠C3，但在派氏布鲁线虫Mf上未检测到。结合的补体促进巨噬细胞和中性粒细胞介导的对这些Mf的黏附和细胞毒性。然而，BALB/C nu/+或nu/nu小鼠的巨噬细胞和中性粒细胞在新鲜正常小鼠血清（NMS）存在的情况下黏附于派氏布鲁线虫脱鞘的Mf并将其杀死。在派氏布鲁线虫和马来布鲁线虫脱鞘Mf的角质层上检测到C3分子。通过加热或酵母聚糖或抗小鼠C3去除补体因子的新鲜NMS（nu/+或nu/nu）未能促进细胞对这些寄生虫的黏附。EDTA而非EGTA消除了对马来布鲁线虫带鞘和脱鞘Mf的黏附活性，而EGTA和EDTA均消除了对派氏布鲁线虫脱鞘Mf和彭亨布鲁线虫带鞘Mf的黏附活性，这表明这些密切相关物种的补体激活途径不同。来自nu/+或nu/nu腹膜渗出细胞的巨噬细胞和中性粒细胞在黏附和杀死Mf方面同样有效。从BALB/C小鼠的腹腔中回收了输入的派氏布鲁线虫Mf，但未回收马来布鲁线虫或彭亨布鲁线虫Mf，且没有细胞黏附和细胞毒性。
