College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, PR China.
Research Center for Animal Disease Control Engineering Shandong Province, Shandong Agricultural University, Tai'an 271018, PR China.
Biomed Pharmacother. 2017 Sep;93:146-155. doi: 10.1016/j.biopha.2017.05.138. Epub 2017 Jun 17.
As a traditional Chinese multiherbal formula, Yu-Ping-Feng (YPF) is frequently used to treat cold, flu and inflammation-associated diseases. We aimed to evaluate the immunostimulatory effects of polysaccharide isolated from YPF (YPF-PS) in vitro and in vivo. In in vitro experiment, macrophage cell proliferation, phagocytosis rate, cytokine and costimulatory molecule release, T lymphocyte proliferation, cell cycle distribution, CD4 and CD8 T cell percentages were determined. To investigate the in vivo effects of YPF-PS treatment, different doses YPF-PS were administered to chicken vaccinated against Newcastle disease. The immune organ index, lymphocyte proliferation, antibody titer, cell cycle distribution, and the cell percentage of CD4 and CD8 were assessed. In vitro results indicated that YPF-PS at 15.62μgmL could increase the LPS-induced macrophage cell proliferation and phagocytosis rate significantly. The levels of cytokine (nitric oxide, tumor necrosis factor-alpha, interleukin-1 beta, interleukin-6, and interferon beta) and costimulatory molecules (CD80 and CD86) were also considerably enhanced. Moreover, YPF-PS could significantly enhance T lymphocyte proliferation individually or synergistically with phytohemagglutinin. It promoted lymphocyte entry into S and G2/M phases and increased the percentages of CD4 and CD8 T cells effectively. In addition, in vivo experiments showed that YPF-PS could enhance serum HI antibody titer. The results about T lymphocyte proliferation, cell cycle distribution, CD4 and CD8 cell percentages in chickens were also confirmed. YPF-PS has efficacious immunomodulatory properties and could be used as a new potential immune stimulator for food and medical purposes.
作为一种传统的中草药复方，玉屏风（YPF）常用于治疗感冒、流感和炎症相关疾病。本研究旨在评估从 YPF 中分离得到的多糖（YPF-PS）在体外和体内的免疫刺激作用。在体外实验中，测定了巨噬细胞增殖、吞噬率、细胞因子和共刺激分子释放、T 淋巴细胞增殖、细胞周期分布、CD4 和 CD8 T 细胞百分比。为了研究 YPF-PS 治疗的体内作用，用不同剂量的 YPF-PS 给接种新城疫疫苗的鸡进行处理。评估免疫器官指数、淋巴细胞增殖、抗体滴度、细胞周期分布以及 CD4 和 CD8 T 细胞的百分比。体外结果表明，15.62μgmL 的 YPF-PS 可显著增加 LPS 诱导的巨噬细胞增殖和吞噬率。细胞因子（一氧化氮、肿瘤坏死因子-α、白细胞介素-1β、白细胞介素-6 和干扰素-β）和共刺激分子（CD80 和 CD86）的水平也显著增强。此外，YPF-PS 可单独或协同植物血凝素显著增强 T 淋巴细胞的增殖。它有效地促进淋巴细胞进入 S 和 G2/M 期，并增加 CD4 和 CD8 T 细胞的百分比。此外，体内实验表明，YPF-PS 可增强血清 HI 抗体滴度。鸡的 T 淋巴细胞增殖、细胞周期分布、CD4 和 CD8 细胞百分比的结果也得到了证实。YPF-PS 具有有效的免疫调节特性，可作为食品和医学用途的新的潜在免疫刺激物。
