Yang Shubao, Shan Chunlan, Ma Xin, Qin Yanjun, Ju Anqi, Duan Aoyi, Luan Weimin, Zhang Yingnan
Center For Reproductive Medicine, Jilin Medical University, Jilin 132013, China; College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China.
College of Animal Science and Technology, Yunnan Agricultural University, Yunnan 650210, China.
Poult Sci. 2021 Feb;100(2):623-630. doi: 10.1016/j.psj.2020.11.059. Epub 2020 Dec 1.
The immunomodulatory effect of Acanthopanax senticosus polysaccharide (ASPS) on immunosuppressed chickens induced by cyclophosphamide (Cy) was observed in this study. Four hundred 7-day-old chickens were randomly divided into 4 groups: vaccinated control group (VC group), Cy-challenged control group (Cy group), Cy-challenged + low-dose ASPS group (ASPS + Cy group), and Cy-challenged + high-dose ASPS group (ASPS + Cy group). All groups except the VC group were injected with Cy at a dose of 80 mg/kg/day of BW for 3 successive days to induce immunosuppression. At the age of 10 d, the ASPS + Cy group and ASPS + Cy group were intramuscularly injected with 0.2 mL of ASPS at the dose of 100 and 200 mg/mL/day, respectively, once a day for 3 successive days. The Cy group was injected with saline solution in the same way as the 2 ASPS groups. At the age of 14 d, the chickens were vaccinated with Newcastle disease (ND) vaccine in all groups. On day 7, 14, 21, and 28 after the vaccination, BW, lymphocyte proliferation, the serum antibody titers of the ND vaccine, the proportion of CD4 and CD8 T lymphocytes, and the concentrations of interferon gamma and IL-2 were determined. The results showed that chickens were injected with Cy at a dose of 80 mg/kg of BW for 3 d displayed lower immune responses than the control group, indicating that the immunosuppressive model was successfully established. At most time points, both high and low doses of ASPS could significantly promote lymphocyte proliferation; enhance BW, antibody titers, and the proportion of CD4 and CD8 T lymphocytes; and raised the concentrations of interferon gamma and IL-2 in Cy-treated chickens compared with those in the Cy control group (P < 0.05). These results indicated that ASPS could resist immunosuppression induced by Cy and may be a new-type immune adjuvant to improve vaccination in normal and immunosuppressed chickens.
本研究观察了刺五加多糖（ASPS）对环磷酰胺（Cy）诱导的免疫抑制鸡的免疫调节作用。将400只7日龄鸡随机分为4组：疫苗接种对照组（VC组）、Cy攻击对照组（Cy组）、Cy攻击+低剂量ASPS组（ASPS+Cy组）和Cy攻击+高剂量ASPS组（ASPS+Cy组）。除VC组外，所有组均以80mg/kg体重/天的剂量连续3天注射Cy以诱导免疫抑制。在10日龄时，ASPS+Cy组和ASPS+Cy组分别以100和200mg/mL/天的剂量肌肉注射0.2mL ASPS，每天1次，连续3天。Cy组以与2个ASPS组相同的方式注射生理盐水。在14日龄时，所有组的鸡均接种新城疫（ND）疫苗。在接种疫苗后的第7、14、21和28天，测定体重、淋巴细胞增殖、ND疫苗的血清抗体滴度、CD4和CD8 T淋巴细胞的比例以及干扰素γ和IL-2的浓度。结果表明，以80mg/kg体重的剂量注射Cy 3天的鸡的免疫反应低于对照组，表明免疫抑制模型成功建立。在大多数时间点，与Cy对照组相比，高剂量和低剂量的ASPS均可显著促进淋巴细胞增殖；提高体重、抗体滴度以及CD4和CD8 T淋巴细胞的比例；并提高Cy处理鸡的干扰素γ和IL-2浓度（P<0.05）。这些结果表明，ASPS可抵抗Cy诱导的免疫抑制，可能是一种新型免疫佐剂，可改善正常和免疫抑制鸡的疫苗接种效果。
