Zhao Xiaona, Sun Wenjing, Zhang Shijie, Meng Guangju, Qi Chunhua, Fan Wentao, Wang Yuge, Liu Jianzhu
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, PR China.
Zhengzhou Ninth People's Hospital, Zhengzhou 450053, PR China.
Carbohydr Polym. 2016 May 5;141:190-6. doi: 10.1016/j.carbpol.2016.01.013. Epub 2016 Jan 12.
Build on our previous research, polysaccharides from the rhizome of Atractylodis macrocephalae Koidz (RAMPS), RAMPStp and RAMPS60c were prepared and the structural characterization and immune response of ND vaccine in chicken were investigated. Immune organ index, Lymphocyte proliferation, antibody titers, cell cycle distribution, and percentages of CD4(+) and CD8(+) cells were determined. GPC analysis showed that the Mn of RAMPS with two peaks were 1.29×10(5) and 1.74×10(3), respectively. GC-MS analysis revealed that RAMPS was composed of glucose, mannose, arabinose, galactose, xylose, d-Ribose and rhamnose, with mass percentages of 66.39%, 21.24%, 5.64%, 2.65%, 2.30%, 1.15% and 0.64%, respectively. NMR spectroscopic analysis demonstrated that a preliminary structure of RAMPS was proposed as 1,3-linked β-d-Galp and 1,6-linked β-d-Galpresidues. In vivo test showed that RAMPStp and RAMPS60c could promote peripheral lymphocytes proliferation and entering into S and G2/M phases, enhance serum HI antibody titer and effectively improve the percentages of CD4(+) and CD8(+) T cells in chickens vaccinated with ND vaccine at most time points. The actions of RAMPStp and RAMPS60c were stronger than that of Lev, and RAMPStp presented the best efficacy. These results indicated that RAMPStp and RAMPS60c characterize of the immune-enhancing activity and RAMPStp possessed the strongest activity. It would be anticipated as a component of new-type immunopotentiator.
基于我们之前的研究，制备了白术根茎多糖（RAMPS）、RAMPStp和RAMPS60c，并研究了其结构特征以及对鸡新城疫疫苗免疫反应的影响。测定了免疫器官指数、淋巴细胞增殖、抗体效价、细胞周期分布以及CD4(+)和CD8(+)细胞的百分比。凝胶渗透色谱分析表明，具有两个峰的RAMPS的数均分子量分别为1.29×10(5)和1.74×10(3)。气相色谱-质谱分析显示，RAMPS由葡萄糖、甘露糖、阿拉伯糖、半乳糖、木糖、d-核糖和鼠李糖组成，质量百分比分别为66.39%、21.24%、5.64%、2.65%、2.30%、1.15%和0.64%。核磁共振光谱分析表明，RAMPS的初步结构被认为是由1,3-连接的β-d-吡喃半乳糖和1,6-连接的β-d-吡喃半乳糖残基组成。体内试验表明，RAMPStp和RAMPS60c可促进外周淋巴细胞增殖并进入S期和G2/M期，提高血清血凝抑制抗体效价，并在大多数时间点有效提高接种新城疫疫苗鸡的CD4(+)和CD8(+) T细胞百分比。RAMPStp和RAMPS60c的作用强于左旋咪唑，且RAMPStp的效果最佳。这些结果表明，RAMPStp和RAMPS60c具有免疫增强活性，且RAMPStp活性最强。有望作为新型免疫增强剂的一个成分。
