Yao Chun-Hsu, Lee Chia-Yu, Huang Chiung-Hua, Chen Yueh-Sheng, Chen Kuo-Yu
Biomaterials Translational Research Center, China Medical University Hospital, Taichung 40202, Taiwan; Department of Biomedical Imaging and Radiological Science, China Medical University, Taichung 40202, Taiwan; School of Chinese Medicine, China Medical University, Taichung 40402, Taiwan; Department of Biomedical Informatics, Asia University, Taichung 41354, Taiwan.
Graduate Institute of Clinical Medical Science, China Medical University, Taichung 40402, Taiwan.
Mater Sci Eng C Mater Biol Appl. 2017 Oct 1;79:533-540. doi: 10.1016/j.msec.2017.05.076. Epub 2017 May 14.
A bilayer membrane (GKU) with a commercial polyurethane wound dressing as an outer layer and electrospun gelatin/keratin nanofibrous mat as an inner layer was fabricated as a novel wound dressing. Scanning electron micrographs showed that gelatin/keratin nanofibers had a uniform morphology and bead-free structure with average fiber diameter of 160.4nm. 3-(4,5-Dimethylthiazolyl)-2,5-diphenyltetrazolium bromide assay using L929 fibroblast cells indicated that the residues released from the gelatin/keratin composite nanofibrous mat accelerated cell proliferation. Cell attachment experiments revealed that adhered cells spread better and migrated deeper into the gelatin/keratin nanofibrous mat than that into the gelatin nanofibrous mat. In animal studies, compared with the bilayer membrane without keratin, gauze and commercial wound dressing, Comfeel®, GKU membrane gave much more number of blood vessels and a greater reduction in wound area at 4days, and better wound repair at 14days with a thicker epidermis and larger number of newly formed hair follicles. GKU membrane, thus, could be a good candidate for wound dressing applications.
制备了一种双层膜（GKU），其外层为商用聚氨酯伤口敷料，内层为静电纺丝明胶/角蛋白纳米纤维垫，作为一种新型伤口敷料。扫描电子显微镜照片显示，明胶/角蛋白纳米纤维形态均匀，无珠状结构，平均纤维直径为160.4nm。使用L929成纤维细胞进行的3-(4,5-二甲基噻唑基)-2,5-二苯基四氮唑溴盐检测表明，从明胶/角蛋白复合纳米纤维垫释放的残留物促进了细胞增殖。细胞附着实验表明，与明胶纳米纤维垫相比，黏附细胞在明胶/角蛋白纳米纤维垫中铺展得更好，迁移得更深。在动物研究中，与不含角蛋白的双层膜、纱布和商用伤口敷料Comfeel®相比，GKU膜在4天时产生的血管数量更多，伤口面积减少得更多，在14天时伤口修复效果更好，表皮更厚，新形成的毛囊数量更多。因此，GKU膜可能是伤口敷料应用的良好候选材料。
