壳聚糖/氧化石墨烯聚合物纳米纤维的制备及其在软骨组织工程中的生物相容性。
Fabrication of chitosan/graphene oxide polymer nanofiber and its biocompatibility for cartilage tissue engineering.
机构信息
Department of Trauma and Orthopedics, Shanghai General Hospital, Shanghai Jiao Tong University, Shanghai 201620, China.
Department of Trauma and Orthopedics, Shanghai General Hospital, Shanghai Jiao Tong University, Shanghai 201620, China.
出版信息
Mater Sci Eng C Mater Biol Appl. 2017 Oct 1;79:697-701. doi: 10.1016/j.msec.2017.05.056. Epub 2017 May 10.
In this work, Chitosan/Poly(vinyl alcohol) (PVA)/graphene oxide (GO) composite nanofibers were fabricated by electrospinning technique. The prepared nanofibers were characterized by using various spectroscopic and microscopic techniques. FESEM images have confirmed the uniform distributions of graphene oxide nanosheets in the nanofibers with self-assembly with chitosan/PVA chains. Additionally, the results of raman spectra confirmed the existence of GO sheets in the nanofibers. The tensile strength experiments revealed that the incorporation of GO increased the mechanical properties of nanofibers. Further the biocompatibilities of the chitosan/PVA/GO towards ATDC5 cells was studied in a cell proliferation assay after day 14. The obtained results revealed that the chitosan/PVA/GO (6wt%) is found to deliver the most appropriate environment for the growth of ATDC5 cells when compared with chitosan/PVA/GO (4wt%) and chitosan/PVA. Hence, the chitosan/PVA/GO (6wt%) nanofiber can be used as possible substitute as an artificial cartilage.
在这项工作中，通过静电纺丝技术制备了壳聚糖/聚乙烯醇（PVA）/氧化石墨烯（GO）复合纳米纤维。采用各种光谱和显微镜技术对制备的纳米纤维进行了表征。FESEM 图像证实了氧化石墨烯纳米片在纳米纤维中的均匀分布，并与壳聚糖/PVA 链进行了自组装。此外，拉曼光谱的结果证实了纳米纤维中 GO 片的存在。拉伸强度实验表明，GO 的加入提高了纳米纤维的机械性能。进一步研究了壳聚糖/PVA/GO 对 ATDC5 细胞的生物相容性，在第 14 天后通过细胞增殖实验进行了研究。结果表明，与壳聚糖/PVA/GO（4wt%）和壳聚糖/PVA 相比，壳聚糖/PVA/GO（6wt%）在为 ATDC5 细胞的生长提供最合适的环境方面表现最佳。因此，壳聚糖/PVA/GO（6wt%）纳米纤维可用作人工软骨的替代品。
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