Murry Reyna, Kniemeyer Olaf, Krause Katrin, Saiardi Adolfo, Kothe Erika
Friedrich Schiller University Jena, Institute of Microbiology, Jena, Germany.
Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute Jena, Germany.
Adv Biol Regul. 2019 May;72:78-88. doi: 10.1016/j.jbior.2019.01.001. Epub 2019 Jan 3.
Mushroom forming basidiomycete Schizophyllum commune has been used as a tractable model organism to study fungal sexual development. Ras signaling activation via G-protein-coupled receptors (GPCRs) has been postulated to play a significant role in the mating and development of S. commune. In this study, a crosstalk between Ras signaling and inositol phosphate signaling by inositol monophosphatase (IMPase) is revealed. Constitutively active Ras1 leads to the repression of IMPase transcription and lithium action on IMPase activity is compensated by the induction of IMPase at transcriptome level. Astonishingly, in S. commune lithium induces a considerable shift to inositol phosphate metabolism leading to a massive increase in the level of higher phosphorylated inositol species up to the inositol pyrophosphates. The lithium induced metabolic changes are not observable in a constitutively active Ras1 mutant. In addition to that, proteome profile helps us to elucidate an overview of lithium action to the broad aspect of fungal metabolism and cellular signaling. Taken together, these findings imply a crosstalk between Ras and inositol phosphate signaling.
形成蘑菇的担子菌裂褶菌已被用作研究真菌有性发育的一种易于处理的模式生物。据推测，通过G蛋白偶联受体（GPCRs）激活Ras信号在裂褶菌的交配和发育中起重要作用。在本研究中，揭示了Ras信号与由肌醇单磷酸酶（IMPase）介导的肌醇磷酸信号之间的相互作用。组成型活性Ras1导致IMPase转录的抑制，并且锂对IMPase活性的作用在转录组水平上通过IMPase的诱导得到补偿。令人惊讶的是，在裂褶菌中，锂诱导肌醇磷酸代谢发生相当大的转变，导致高达肌醇焦磷酸的更高磷酸化肌醇种类水平大幅增加。在组成型活性Ras1突变体中未观察到锂诱导的代谢变化。除此之外，蛋白质组图谱有助于我们阐明锂对真菌代谢和细胞信号广泛方面作用的概况。综上所述，这些发现暗示了Ras和肌醇磷酸信号之间的相互作用。
