Poordast Tahereh, Najib Fateme Sadat, Baharlou Rasoul, Bijani Atena, Alamdarloo Shaghayegh Moradi, Poordast Alieh
Infertility Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Iran J Immunol. 2017 Jun;14(2):172-179.
Preeclampsia is a common pregnancy-specific disorder associated with significant maternal and fetal morbidity and mortality worldwide. It has been proposed that the imbalance between two CD4+ T cell subtypes, regulatory T cells (Treg) and T-helper 17 cells (Th17), is involved in the pathophysiology of preeclampsia.
To determine the serum levels of IL-17, IL-21, IL-23 and TGF-β in patients with preeclampsia.
Blood samples were collected from 30 preeclampsia patients, 30 normotensive pregnant women and 30 healthy individuals with no history of malignancies or autoimmune disorders based on simple sampling. The serum levels of IL-17, IL-21, IL-23 and TGF-β were measured by the enzyme linked immunosorbent assay (ELISA).
The serum levels of IL-17 and TGF-β were significantly higher in preeclampsia patients compared to normal pregnant group and healthy individuals (p>0.0001) but interestingly, the opposite was the case for IL-23 (p=0.005). However, there were no significant differences in IL-21 between preeclampsia and normal pregnant group.
Our results conclude that contrary to IL-21, serum levels of IL-17 and TGF-β significantly increased in preeclampsia compared to normal pregnant women, supporting an imbalance of cytokine profile in preeclamtic patients.
子痫前期是一种常见的妊娠特异性疾病，在全球范围内与孕产妇和胎儿的高发病率及死亡率相关。有人提出，两种CD4 + T细胞亚型，即调节性T细胞（Treg）和辅助性T17细胞（Th17）之间的失衡参与了子痫前期的病理生理过程。
测定子痫前期患者血清中白细胞介素-17（IL-17）、白细胞介素-21（IL-21）、白细胞介素-23（IL-23）和转化生长因子-β（TGF-β）的水平。
采用简单抽样法，从30例子痫前期患者、30例血压正常的孕妇和30例无恶性肿瘤或自身免疫性疾病病史的健康个体中采集血样。采用酶联免疫吸附测定法（ELISA）检测血清中IL-17、IL-21、IL-23和TGF-β的水平。
与正常妊娠组和健康个体相比，子痫前期患者血清中IL-17和TGF-β水平显著升高（p>0.0001），但有趣的是，IL-23的情况则相反（p = 0.005）。然而，子痫前期组与正常妊娠组之间IL-21水平无显著差异。
我们的结果表明，与IL-21相反，子痫前期患者血清中IL-17和TGF-β水平相比正常孕妇显著升高，这支持子痫前期患者细胞因子谱失衡。
