Wells R M, Davie P S
Comp Biochem Physiol A Comp Physiol. 1985;81(3):643-6. doi: 10.1016/0300-9629(85)91041-2.
Blood from capture-stressed striped marlin cannot be fully saturated with oxygen in the presence of lactic acid because of a substantial Root effect. Titration of blood to pH 7.6 at 25 degrees C gave an O2 affinity constant, P50 = 17 mmHg. The P50 was highly sensitive to pH showing a Bohr factor phi = delta log P50/delta pH = -0.74. Blood from capture-stressed mako sharks did not show either a Root effect or a significant Bohr effect (delta log P50/delta pH = +0.16). The O2-affinity constant, P50, was 10.6 mmHg at pH 7.6 and 25 degrees C. Hematological measurements following capture gave hematocrit values of 24.2 +/- 8.1% and 32.3 +/- 10.8% for marlin and mako respectively, and high concentrations of blood lactate in both species (15-16 mmol l-1). The O2 transport properties of the blood are discussed in relation to oxygen demand and capture stress.
由于显著的鲁特效应，捕获应激状态下条纹枪鱼的血液在乳酸存在时无法完全被氧气饱和。在25摄氏度下将血液滴定至pH 7.6时，氧气亲和力常数P50 = 17 mmHg。P50对pH高度敏感，玻尔因子phi = Δlog P50/ΔpH = -0.74。捕获应激状态下的灰鲭鲨血液既没有显示出鲁特效应，也没有显著的玻尔效应（Δlog P50/ΔpH = +0.16）。在pH 7.6和25摄氏度时，氧气亲和力常数P50为10.6 mmHg。捕获后的血液学测量显示，枪鱼和灰鲭鲨的血细胞比容值分别为24.2±8.1%和32.3±10.8%，两种鱼的血液乳酸浓度都很高（15 - 16 mmol·l-1）。本文讨论了血液的氧气运输特性与氧气需求和捕获应激的关系。
