Zhang Huicun, Ta Na, Chen Pengmin, Wang Hongbing
Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, China.
Beijing Institute of Traditional Chinese Medicine, Beijing 100010, China.
Evid Based Complement Alternat Med. 2017;2017:1589871. doi: 10.1155/2017/1589871. Epub 2017 May 29.
Erchen decoction (ECD) and Linguizhugan decoction (LGZGD), both are Chinese herbal formula, have been used clinically for the treatment of nonalcoholic fatty liver disease (NAFLD). However, their therapeutic mechanisms are still unclear. Because insulin resistance (IR) is a key etiological factor in the pathology of high-fat diet- (HFD-) induced NAFLD, in this study, the protective effects of ECD and LGZGD on HFD-induced insulin resistance in rats were evaluated and their mechanisms were investigated by OGTT and Western blot. The results showed that treatment with ECD and LGZGD significantly improved insulin resistance and liver damage in rats, evidenced by supported serum aminotransferase levels and the histopathological examination. ECD and LGZGD also showed significant protective effects against HFD-induced hyperlipidemia and the inhibition of the hepatocyte proliferation by palmitate. Furthermore, supplementation of ECD and LGZGD decreased TNF-, NF-B, and IRS-1Ser307 phosphorylation expressions in vivo and in vitro. These results indicated that ECD and LGZGD have protective effects against HFD-induced liver IR and their underlying mechanisms involve the TNF- and insulin pathway. These findings would be beneficial for understanding of the therapeutic effects of ECD and LGZGD in treatment of NAFLD.
二陈汤（ECD）和苓桂术甘汤（LGZGD）均为中药方剂，已在临床上用于治疗非酒精性脂肪性肝病（NAFLD）。然而，它们的治疗机制仍不清楚。由于胰岛素抵抗（IR）是高脂饮食（HFD）诱导的NAFLD发病机制中的关键病因，在本研究中，通过口服葡萄糖耐量试验（OGTT）和蛋白质免疫印迹法（Western blot）评估了ECD和LGZGD对HFD诱导的大鼠胰岛素抵抗的保护作用，并对其机制进行了研究。结果表明，ECD和LGZGD治疗显著改善了大鼠的胰岛素抵抗和肝损伤，血清转氨酶水平及组织病理学检查结果支持这一结论。ECD和LGZGD对HFD诱导的高脂血症以及棕榈酸对肝细胞增殖的抑制也显示出显著的保护作用。此外，补充ECD和LGZGD可降低体内和体外肿瘤坏死因子-α（TNF-α）、核因子-κB（NF-κB）以及胰岛素受体底物-1丝氨酸307（IRS-1Ser307）的磷酸化表达。这些结果表明，ECD和LGZGD对HFD诱导的肝脏IR具有保护作用，其潜在机制涉及TNF-α和胰岛素信号通路。这些发现将有助于理解ECD和LGZGD在治疗NAFLD中的疗效。
