Zhen Gao, Upur Halmurat, Jing Wang, Jing Jing, Zheng Li, Dan Xu, Fengsen Li
National Clinical Research Base of Traditional Chinese Medicine, Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical University, Urumqi 830000, China.
Evid Based Complement Alternat Med. 2017;2017:7176263. doi: 10.1155/2017/7176263. Epub 2017 May 29.
To investigate the effect of abnormal savda munziq (ASM) on the pulmonary function and expression of lung-specific aquaporins in the rat model of chronic obstructive pulmonary disease with abnormal savda syndrome (ASSCOPD).
Eighty male rats were randomized into ASSCOPD, COPD, and control groups. ASSCOPD was further categorized into ASM and non-ASM groups. COPD model was established by combining fumigation with airway instillation of elastase; ASSCOPD model was developed based on COPD by induction with dry cold diet, cold dry environment, and plantar electric stimulation. ASM was administered twice daily. The pulmonary function was evaluated based on respiration. The mRNA and protein levels of AQPs were estimated by real-time PCR and Western blot, respectively.
MV, TV, the mRNA level of AQP5, and the protein expression of AQP1, AQP4, and AQP5 were increased in ASMCOPD compared to ASSCOPD.
The pulmonary function was impaired in ASSCOPD group; the expression of AQP1, AQP4, and AQP5 was decreased at protein and mRNA levels in ASSCOPD group. ASM can improve the pulmonary function in ASSCOPD for MV and TV. ASM could elevate the protein expression of AQP1, AQP4, and AQP5 and the mRNA level of AQP5 in lung tissue.
探讨异常黑胆质成熟剂（ASM）对异常黑胆质证型慢性阻塞性肺疾病（ASSCOPD）大鼠模型肺功能及肺特异性水通道蛋白表达的影响。
将80只雄性大鼠随机分为ASSCOPD组、COPD组和对照组。ASSCOPD组进一步分为ASM组和非ASM组。采用烟熏联合气道内滴注弹性蛋白酶建立COPD模型；在COPD模型基础上，通过给予干冷饮食、冷干环境及足底电刺激诱导建立ASSCOPD模型。ASM每日给药2次。根据呼吸情况评估肺功能。分别采用实时荧光定量PCR和蛋白质印迹法检测水通道蛋白（AQPs）的mRNA和蛋白水平。
与ASSCOPD组相比，ASM-COPD组的分钟通气量（MV）、潮气量（TV）、水通道蛋白5（AQP5）的mRNA水平以及水通道蛋白1（AQP1）、水通道蛋白4（AQP4）和AQP5的蛋白表达均升高。
ASSCOPD组肺功能受损；ASSCOPD组AQP1、AQP4和AQP5的蛋白及mRNA水平表达降低。ASM可改善ASSCOPD组的MV和TV肺功能。ASM可提高肺组织中AQP1、AQP4和AQP5的蛋白表达以及AQP5的mRNA水平。
