血清干血斑样本检测登革热抗体：一项现场研究。
Serum Dried Samples to Detect Dengue Antibodies: A Field Study.
机构信息
Unidad de Investigacion Medica en Enfermedades Infecciosas y Parasitarias, UMAE Hospital de Pediatria, Centro Medico Nacional "Siglo XXI", Instituto Mexicano del Seguro Social (IMSS), Mexico City, Mexico.
Educacion e Investigacion en Salud, UMAE Hospital de Especialidades, Centro Medico Nacional "Siglo XXI", Instituto Mexicano del Seguro Social (IMSS), Mexico City, Mexico.
出版信息
Biomed Res Int. 2017;2017:7215259. doi: 10.1155/2017/7215259. Epub 2017 May 29.
BACKGROUND
Dried blood and serum samples are useful resources for detecting antiviral antibodies. The conditions for elution of the sample need to be optimized for each disease. Dengue is a widespread disease in Mexico which requires continuous surveillance. In this study, we standardized and validated a protocol for the specific detection of dengue antibodies from dried serum spots (DSSs).
METHODS
Paired serum and DSS samples from 66 suspected cases of dengue were collected in a clinic in Veracruz, Mexico. Samples were sent to our laboratory, where the conditions for optimal elution of DSSs were established. The presence of anti-dengue antibodies was determined in the paired samples.
RESULTS
DSS elution conditions were standardized as follows: 1 h at 4°C in 200 l of DNase-, RNase-, and protease-free PBS (1x). The optimal volume of DSS eluate to be used in the IgG assay was 40 l. Sensitivity of 94%, specificity of 93.3%, and kappa concordance of 0.87 were obtained when comparing the antidengue reactivity between DSSs and serum samples.
CONCLUSION
DSS samples are useful for detecting anti-dengue IgG antibodies in the field.
背景
干血和血清样本是检测抗病毒抗体的有用资源。对于每种疾病，都需要优化样本洗脱的条件。登革热是墨西哥广泛存在的疾病，需要持续监测。在本研究中，我们标准化并验证了一种从干血清斑（DSS）中特异性检测登革热抗体的方案。
方法
在墨西哥韦拉克鲁斯的一家诊所收集了 66 例疑似登革热的血清和 DSS 配对样本。样本被送到我们的实验室，在那里确定了 DSS 的最佳洗脱条件。在配对样本中检测抗登革热抗体的存在。
结果
将 DSS 洗脱条件标准化为：4°C 下用 200μl 无 DNase、无 RNase 和无蛋白酶的 PBS（1x）孵育 1 小时。在 IgG 检测中，DSS 洗脱液的最佳使用体积为 40μl。当比较 DSS 与血清样本之间的抗登革热反应时，获得了 94%的敏感性、93.3%的特异性和 0.87 的kappa 一致性。
结论
DSS 样本可用于现场检测抗登革热 IgG 抗体。
Zotero 插件