Yu Yongzhong, Tan Qiang, Zhao Wenbo, Zhang Xue, Ma Jinzhu, Wu Zhijun, Zhu Zhanbo, Cui Yudong
Virology Laboratory, College of Biological Science and Technology, Heilongjiang Bayi Agricultural University, 2 Xinyang road, Daqing, 163319, China.
Virology Laboratory, Division of Animal Health, College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, 2 Xinyang road, Daqing, 163319, China.
Arch Virol. 2017 Oct;162(10):3143-3149. doi: 10.1007/s00705-017-3426-x. Epub 2017 Jun 19.
Contagious ecthyma, caused by orf virus (ORFV), is an epitheliotrophic contagious disease with zoonotic implications that mainly affects sheep, goats, wild ruminants, and humans. Recently, a novel ORFV strain, OV/HLJ/04, was successfully isolated from the skin and mucosal lesions of a goat with severe clinical sore mouth symptoms in Heilongjiang province of China. The OV/HLJ/04 isolate was characterized by electron microscopy, serological tests, and experimental reproduction of disease. The purified virions exhibited a typical ovoid shape when observed by electron microscopy. Moreover, experimental reproduction of disease showed that a lamb developed typical clinical signs of contagious ecthyma, such as severe vascular proliferation, when inoculated with the virus. Subsequently, amplification of ORFV011 (B2L) gene fragments of viral DNA by polymerase chain reaction (PCR) and gene sequencing were performed. Phylogenetic analysis of the B2L protein gene revealed that this strain clusters with ORFV strains from epidemic-stricken areas worldwide, including recent mainland China isolates. Analysis using ClustalW MegAlign in DNAStar indicated that OV/HLJ/04 (GenBank: KU523790.1) was genetically closely related to the isolates Gansu (JQ904789), with 99.7% identity; NZ2 (DQ184476), with 97.4% identity; and Xinjiang (KF666560), with 90.6% identity. These results may provide insights into the genotype of the etiological agent responsible for the orf outbreak in Heilongjiang Province.
由口疮病毒（ORFV）引起的传染性脓疱性皮炎是一种具有人畜共患病影响的嗜上皮性传染病，主要影响绵羊、山羊、野生反刍动物和人类。最近，在中国黑龙江省，从一只患有严重临床口疮症状的山羊的皮肤和黏膜病变中成功分离出一种新型ORFV毒株OV/HLJ/04。通过电子显微镜、血清学检测和疾病实验复制对OV/HLJ/04分离株进行了鉴定。通过电子显微镜观察，纯化的病毒粒子呈现出典型的卵形。此外，疾病实验复制表明，一只羔羊接种该病毒后出现了传染性脓疱性皮炎的典型临床症状，如严重的血管增生。随后，通过聚合酶链反应（PCR）对病毒DNA的ORFV011（B2L）基因片段进行扩增并进行基因测序。对B2L蛋白基因的系统发育分析表明，该毒株与来自全球疫区的ORFV毒株聚集在一起，包括中国大陆最近的分离株。使用DNAStar中的ClustalW MegAlign进行分析表明，OV/HLJ/04（GenBank：KU523790.1）与甘肃分离株（JQ904789）在基因上密切相关，同一性为99.7%；与NZ2（DQ184476）同一性为97.4%；与新疆分离株（KF666560）同一性为90.6%。这些结果可能为黑龙江省口疮疫情病原体的基因型提供见解。
