Kanuni Sultan Suleyman Training and Research Hospital, Department of Orthopaedic Surgery, University of Health Sciences, Turgut Ozal Street, Altinsehir/Kucukcekmece, 34303, Istanbul, Turkey.
Department of Orthopaedic Surgery, Harran University Faculty of Medicine, Sanliurfa, Turkey.
Clin Rheumatol. 2017 Dec;36(12):2775-2780. doi: 10.1007/s10067-017-3732-4. Epub 2017 Jun 19.
Previous in vitro studies have shown that oxidized low-density lipoprotein (ox-LDL) plays a role in the pathogenesis of osteoarthritis (OA). Paraoxonase-1 (PON1) protects both low-density lipoproteins (LDLs) and high-density lipoprotein (HDLs) against oxidative damage from circulating cells. In addition, PON1 is inactivated by ox-LDL and preserved by antioxidants. However, the relationship between serum ox-LDL, oxidative stress, and PON1 in knee OA remains unclear. Therefore, we investigated ox-LDL association with oxidative stress and PON1 in knee OA, and evaluated their relationships using radiological and clinical parameters. This study included 203 patients and 194 controls. The severity of OA was classified based on the Kellgren-Lawrence scoring system. In addition, each patient was clinically evaluated using the Western Ontario and McMaster University Osteoarthritis Index (WOMAC) score. Plasma concentrations of ox-LDL, oxidative stress markers, and PON1 were measured. Serum ox-LDL and oxidant parameters were significantly higher in patients compared to controls (p < 0.001 for all), whereas PON1 was significantly lower (p < 0.001). ox-LDL was inversely correlated with PON1, whereas it was positively correlated with radiographic severity, WOMAC score, and oxidant parameters. We found an association between the levels of various serum markers of oxidative injury, especially ox-LDL, and increasing severity of knee OA, as well as indirect evidence for their regulation by PON1. oxLDL seems to play a critical role in OA, both in the beginning, and during progression of, the disease. Therefore, serum oxLDL levels may be a helpful biomarker to evaluate the severity of knee OA.
先前的体外研究表明，氧化型低密度脂蛋白（ox-LDL）在骨关节炎（OA）发病机制中发挥作用。对氧磷酶-1（PON1）可保护低密度脂蛋白（LDL）和高密度脂蛋白（HDL）免受循环细胞的氧化损伤。此外，PON1 可被 ox-LDL 失活，也可被抗氧化剂保存。然而，血清 ox-LDL、氧化应激和 PON1 与膝骨关节炎之间的关系尚不清楚。因此，我们研究了 ox-LDL 与膝骨关节炎患者的氧化应激和 PON1 的关系，并使用放射学和临床参数评估了它们之间的关系。这项研究包括 203 名患者和 194 名对照。OA 的严重程度基于 Kellgren-Lawrence 评分系统进行分类。此外，每位患者均通过 Western Ontario 和 McMaster 大学骨关节炎指数（WOMAC）评分进行临床评估。测量了 ox-LDL、氧化应激标志物和 PON1 的血浆浓度。与对照组相比，患者的血清 ox-LDL 和氧化剂参数明显升高（所有参数均 p < 0.001），而 PON1 明显降低（p < 0.001）。ox-LDL 与 PON1 呈负相关，而与放射学严重程度、WOMAC 评分和氧化剂参数呈正相关。我们发现，各种血清氧化损伤标志物，尤其是 ox-LDL 的水平与膝骨关节炎严重程度的增加之间存在关联，并且间接证明它们受 PON1 的调节。oxLDL 似乎在 OA 中发挥关键作用，无论是在疾病的早期还是进展期。因此，血清 oxLDL 水平可能是评估膝骨关节炎严重程度的有用生物标志物。
