Fourth Department of Internal Medicine, First Faculty of Medicine, Charles University in Prague and General University Hospital Prague, Czech Republic.
Pancreas. 2013 May;42(4):614-21. doi: 10.1097/MPA.0b013e318288360a.
Oxidative stress has been implicated in the pathogenesis of chronic pancreatitis (CP) and pancreatic cancer (PC). The study aim was to assess the oxidative stress markers and antioxidant defense system in patients with CP and those with PC.
Activities of superoxide dismutase 1 (SOD1), catalase (CAT), glutathione peroxidase 1 (GPX1), glutathione reductase (GR), arylesterase (PON1-A) and lactonase (PON1-L) activities of paraoxonase 1 (PON1) and concentrations of reduced glutathione, conjugated dienes in low-density lipoprotein (CD/LDL) and oxidized LDL (ox-LDL/LDL) were assessed in 50 PC and 50 CP patients and 50 age and sex-matched controls.
Comparison of PC and CP groups to controls found the following changes: glutathione peroxidase 1 (GPX1) (-20.2%, -25.5%; P < 0.001), glutathione reductase (GR) (-9.5%, -11.9%; P < 0.05), SOD1 (+22.9%; P < 0.01), CAT (-10.6%; P < 0.05), PON1-A (-34.3%, -16.0%; P < 0.001), PON1-L (-44.2%; -17.0%; P < 0.01), conjugated dienes in LDL (CD/LDL) (+20%, +33.3%; P < 0.05) and ox-LDL/LDL (+42.2%, +14.4%; P < 0.05). The patients with PC had changed activities and levels of SOD1 (+24.2%), CAT (-10.4); P < 0.01), PON1-A (-21.7%), PON1-L (-32.9%), and ox-LDL/LDL (+24.3%); (all P < 0.01) compared with the patients with CP.
Reduced antioxidant defense system capacity and increased markers of oxidative stress were found in PC and CP. PON1-L and CAT activities, along with ox-LDL/LDL levels, were the independent factors differentiating the patients with PC from the patients with CP.
氧化应激与慢性胰腺炎（CP）和胰腺癌（PC）的发病机制有关。本研究旨在评估 CP 和 PC 患者的氧化应激标志物和抗氧化防御系统。
评估 50 例 PC 患者、50 例 CP 患者和 50 例年龄和性别匹配的对照组的超氧化物歧化酶 1（SOD1）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶 1（GPX1）、谷胱甘肽还原酶（GR）、芳酯酶（PON1-A）和乳过氧化物酶（PON1-L）的活性、还原型谷胱甘肽浓度、低密度脂蛋白（LDL）中的共轭二烯（CD/LDL）和氧化型 LDL（ox-LDL/LDL）。
与对照组相比，PC 组和 CP 组有以下变化：谷胱甘肽过氧化物酶 1（GPX1）（-20.2%，-25.5%；P < 0.001）、谷胱甘肽还原酶（GR）（-9.5%，-11.9%；P < 0.05）、SOD1（+22.9%；P < 0.01）、CAT（-10.6%；P < 0.05）、PON1-A（-34.3%，-16.0%；P < 0.001）、PON1-L（-44.2%；-17.0%；P < 0.01）、LDL 中的共轭二烯（CD/LDL）（+20%，+33.3%；P < 0.05）和 ox-LDL/LDL（+42.2%，+14.4%；P < 0.05）。与 CP 患者相比，PC 患者的 SOD1（+24.2%）、CAT（-10.4）；P < 0.01）、PON1-A（-21.7%）、PON1-L（-32.9%）和 ox-LDL/LDL（+24.3%）的活性和水平发生了变化（均 P < 0.01）。
CP 和 PC 患者的抗氧化防御系统能力降低，氧化应激标志物增加。PON1-L 和 CAT 活性以及 ox-LDL/LDL 水平是区分 PC 患者和 CP 患者的独立因素。
