Department of Orthopaedic Surgery, University of Health Sciences, Hamidiye Faculty of Medicine, Kanuni Sultan Suleyman Training and Research Hospital, Kucukcekmece, Istanbul, Türkiye.
Department of Clinical Biochemistry, Yildirim Beyazit University School of Medicine, Ankara, Türkiye.
Acta Orthop Traumatol Turc. 2024 Jun 27;58(3):161-166. doi: 10.5152/j.aott.2024.22030.
We investigated oxidative status in patients with rotator cuff tendinopathy (RCT) and evaluated their relationship with radiological and clinical parameters. In this cross-section study, 88 patients with RCT (59 males and 29 females) and 86 healthy controls (66 males, 20 females) were enrolled. The sample consisted of nontraumatic patients who are suffering from shoulder pain because of rotator cuff disease, which was established by clinical tests and MRI scanning. Oxidative stress in patients with RCT was analyzed via the dynamic thiol/disulfide homeostasis (TDH). Thiol/disulfide homeostasis was measured by a new colorimetric method. Furthermore, oxidative stress was indirectly measured by serum total oxidant status (TOS), oxidative stress index (OSI), and total antioxidant capacity (TAC). Serum disulfide levels and the other oxidative stress parameters of the RCT group were significantly greater than those of the control group (P < .001 for all), whereas the anti-oxidative stress parameters remained unchanged (P > .05 for all). The lowest and highest serum disulfide levels were detected in patients with grades 1 and 3, respectively (P < .001). Furthermore, in a multiple regression analysis, the disulfide/natural thiol ratio (β=-4.886, P = .004) and the MRI grading (β=0.314, P=.001) were independently associated with the Western Ontario Rotator Cuff Index WORC score. We found an association between the levels of various serum markers of oxidative injury, especially serum disulfide levels, and the increasing severity of RCT. Thiol/disulfide homeostasis seems to play a critical role in RCT, both in the beginning and during the progression of disease.
我们研究了肩袖肌腱病（RCT）患者的氧化状态，并评估了其与影像学和临床参数的关系。在这项横断面研究中，共纳入 88 例 RCT 患者（59 名男性和 29 名女性）和 86 名健康对照者（66 名男性，20 名女性）。该样本由患有肩袖疾病的非创伤性肩部疼痛患者组成，这些患者通过临床检查和 MRI 扫描确定。通过动态硫醇/二硫键动态平衡（TDH）分析 RCT 患者的氧化应激。硫醇/二硫键平衡通过新的比色法测量。此外，通过血清总氧化剂状态（TOS）、氧化应激指数（OSI）和总抗氧化能力（TAC）间接测量氧化应激。RCT 组的血清二硫化物水平和其他氧化应激参数明显大于对照组（所有 P <.001），而抗氧化应激参数保持不变（所有 P >.05）。在 1 级和 3 级患者中分别检测到最低和最高的血清二硫化物水平（P <.001）。此外，在多元回归分析中，二硫化物/天然硫醇比（β=-4.886，P =.004）和 MRI 分级（β=0.314，P =.001）与 Western Ontario Rotator Cuff Index WORC 评分独立相关。我们发现各种血清氧化损伤标志物的水平，尤其是血清二硫化物水平，与 RCT 的严重程度增加之间存在关联。硫醇/二硫键动态平衡似乎在 RCT 的发生和进展中都起着关键作用。
