Arefin Badrul, Kucerova Lucie, Dobes Pavel, Markus Robert, Strnad Hynek, Wang Zhi, Hyrsl Pavel, Zurovec Michal, Theopold Ulrich
Department of Molecular Biosciences, Wenner-Gren Institute, Stockholm University, Stockholm, Sweden.
J Innate Immun. 2014;6(2):192-204. doi: 10.1159/000353734. Epub 2013 Aug 27.
Heterorhabditis bacteriophora is an entomopathogenic nematode (EPN) which infects its host by accessing the hemolymph where it releases endosymbiotic bacteria of the species Photorhabdus luminescens. We performed a genome-wide transcriptional analysis of the Drosophila response to EPN infection at the time point at which the nematodes reached the hemolymph either via the cuticle or the gut and the bacteria had started to multiply. Many of the most strongly induced genes have been implicated in immune responses in other infection models. Mapping of the complete set of differentially regulated genes showed the hallmarks of a wound response, but also identified a large fraction of EPN-specific transcripts. Several genes identified by transcriptome profiling or their homologues play protective roles during nematode infections. Genes that positively contribute to controlling nematobacterial infections encode: a homolog of thioester-containing complement protein 3, a basement membrane component (glutactin), a recognition protein (GNBP-like 3) and possibly several small peptides. Of note is that several of these genes have not previously been implicated in immune responses.
嗜菌异小杆线虫是一种昆虫病原线虫，它通过进入血淋巴来感染宿主，并在血淋巴中释放发光光杆状菌的内共生菌。我们对线虫通过表皮或肠道到达血淋巴且细菌开始繁殖的时间点，进行了全基因组转录分析，以研究果蝇对嗜菌异小杆线虫感染的反应。许多诱导最强烈的基因在其他感染模型的免疫反应中发挥作用。对全套差异调节基因的图谱分析显示出伤口反应的特征，但也鉴定出了很大一部分嗜菌异小杆线虫特异性转录本。通过转录组分析鉴定的几个基因或其同源物在感染线虫期间发挥保护作用。对线虫 - 细菌感染控制有积极作用的基因编码：含硫酯补体蛋白3的同源物、一种基底膜成分（谷胶蛋白）、一种识别蛋白（类GNBP 3）以及可能的几种小肽。值得注意的是，这些基因中有几个以前并未涉及免疫反应。
