Thatte V, Bradley D E, Iyer V N
J Bacteriol. 1985 Sep;163(3):1229-36. doi: 10.1128/jb.163.3.1229-1236.1985.
The mating and plasmid DNA transfer functions in Escherichia coli K-12 strains that are determined by the IncN group plasmid pCU1 are specified by a single 19.4-kilobase (kb) segment of the 39-kb plasmid. Analysis of this tra region by transposon Tn5 mutagenesis and by complementation tests indicated that a subset of tra comprising six complementation groups (presumably transcription units) determines sensitivity to the N pilus-specific bacteriophages IKe and PRD1. Deletion derivatives and molecular clones that contained only this part of tra conferred sensitivity to IKe and PRD1 and the ability to produce N pili without conferring the Tra phenotype. The entire tra region contained two other complementation groups. If the 19.4-kb tra region is interrupted by DNA that is irrelevant to the Tra phenotype, such DNA must be less than 2 kb long. There are two regions within tra where this is a possibility. These observations are compared with those obtained recently with a related plasmid.
由IncN组质粒pCU1决定的大肠杆菌K-12菌株中的交配和质粒DNA转移功能，由39kb质粒的一个19.4千碱基（kb）的单一区段所规定。通过转座子Tn5诱变和互补试验对这个tra区域进行分析表明，tra的一个子集包括六个互补组（推测为转录单位）决定了对N菌毛特异性噬菌体IKe和PRD1的敏感性。仅包含tra这一部分的缺失衍生物和分子克隆赋予了对IKe和PRD1的敏感性以及产生N菌毛的能力，但不赋予Tra表型。整个tra区域还包含另外两个互补组。如果19.4kb的tra区域被与Tra表型无关的DNA中断，那么这种DNA的长度必须小于2kb。在tra内有两个区域存在这种可能性。将这些观察结果与最近用一个相关质粒获得的结果进行了比较。
