结直肠癌特异性抗原：通过单克隆抗体进行检测
Colorectal carcinoma-specific antigen: detection by means of monoclonal antibodies.
作者信息
Herlyn M, Steplewski Z, Herlyn D, Koprowski H
出版信息
Proc Natl Acad Sci U S A. 1979 Mar;76(3):1438-42. doi: 10.1073/pnas.76.3.1438.
Abstract
Fusion of P3 X 63 Ag8 mouse myeloma cells with splenocytes obtained from mice immunized with cells derived from human colorectal carcinomas resulted in the production of antibody-secreting hybridomas. Two hybridomas (1083-17 and 1116-56) and their clones secreted antibodies binding specifically to human colorectal carcinoma cells either grown in culture or obtained from patients, but did not bind to normal colonic mucosa or other normal and malignant human cells. The binding specificity was consistent in three assays: radioimmunoassay, mixed hemadsorption, and immunofluorescence. Adsorption of these antibodies to colorectal carcinoma cell lines totally eliminated their specific binding.
摘要
将P3 X 63 Ag8小鼠骨髓瘤细胞与用源自人结肠直肠癌的细胞免疫的小鼠的脾细胞融合，产生了分泌抗体的杂交瘤。两种杂交瘤（1083 - 17和1116 - 56）及其克隆分泌的抗体可特异性结合培养的或取自患者的人结肠直肠癌细胞，但不与正常结肠黏膜或其他正常和恶性人细胞结合。在放射免疫测定、混合血细胞吸附和免疫荧光这三种测定中，结合特异性是一致的。这些抗体与结肠直肠癌细胞系的吸附完全消除了它们的特异性结合。
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本文引用的文献
1
Detection of antigens in tissue culture with the aid of mixed hemadsorption.借助混合血细胞吸附法检测组织培养中的抗原。
Acta Pathol Microbiol Scand Suppl. 1962;Suppl 154:258-62.
2
Cross-reacting tumor-associated antigen (s) among chemically induced rat colon carcinomas.
Cancer Res. 1974 Aug;34(8):1801-7.
3
Cloning of immunoglobulin-producing human leukemic and lymphoma cells in long-term cultures.长期培养中产生免疫球蛋白的人白血病和淋巴瘤细胞的克隆
Proc Soc Exp Biol Med. 1967 Jan;124(1):107-11. doi: 10.3181/00379727-124-31677.
4
Continuous cultures of fused cells secreting antibody of predefined specificity.分泌预定义特异性抗体的融合细胞的连续培养物。
Nature. 1975 Aug 7;256(5517):495-7. doi: 10.1038/256495a0.
5
Sequential studies of serum blocking activity in rats bearing chemically induced primary bowel tumors.对患有化学诱导原发性肠肿瘤的大鼠血清阻断活性的系列研究。
J Natl Cancer Inst. 1975 Apr;54(4):959-67.
6
Cell surface antigens of human malignant melanoma: mixed hemadsorption assays for humoral immunity to cultured autologous melanoma cells.人类恶性黑色素瘤的细胞表面抗原：针对培养的自体黑色素瘤细胞体液免疫的混合血细胞吸附试验
Proc Natl Acad Sci U S A. 1976 Sep;73(9):3278-82. doi: 10.1073/pnas.73.9.3278.
7
Classification of human colorectal adenocarcinoma cell lines.人类结肠直肠腺癌细胞系的分类。
Cancer Res. 1976 Dec;36(12):4562-9.
8
Lymphocyte cytotoxicity against autologous tumour biopsy cells in humans.人类淋巴细胞对自体肿瘤活检细胞的细胞毒性。
Int J Cancer. 1977 Oct 15;20(4):512-9. doi: 10.1002/ijc.2910200407.
9
Immunology and immunotherapy of colorectal cancer.
Can J Surg. 1978 May;21(3):212-3.
10
Production of antibodies against influenza virus by somatic cell hybrids between mouse myeloma and primed spleen cells.通过小鼠骨髓瘤细胞与致敏脾细胞之间的体细胞杂交产生抗流感病毒抗体。
Proc Natl Acad Sci U S A. 1977 Jul;74(7):2985-8. doi: 10.1073/pnas.74.7.2985.
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