Brunso-Bechtold J K, Hamburger V
Proc Natl Acad Sci U S A. 1979 Mar;76(3):1494-6. doi: 10.1073/pnas.76.3.1494.
We have demonstrated retrograde transport of nerve growth factor to the dorsal root ganglia in the chicken embryo. Polyacrylamide gel pellets impregnated with 125I-labeled nerve growth factor were implanted subcutaneously in the leg of stage 36 (10-day) chicken embryos. The embryos were killed 8 hr after implantation and were processed for routine autoradiography. Examination revealed significant labeling of all lumbar dorsal root ganglia on the side of the implanted pellet. The lateral motor columns and ventral roots as well as the dorsal roots on both sides were unlabeled. These observations show that in the chicken embryo, during the period of sensory cell sensitivity to nerve growth factor, there is a selective uptake and retrograde transport of nerve growth factor from the target area to the dorsal root ganglia. These observations supplement reports from other laboratories in which selective retrograde transport of nerve growth factor in neonatal and adult rodents has been demonstrated.
我们已经证明神经生长因子在鸡胚中能逆向运输至背根神经节。将浸有125I标记神经生长因子的聚丙烯酰胺凝胶小球皮下植入36期（10日龄）鸡胚的腿部。植入后8小时处死胚胎，并进行常规放射自显影处理。检查发现植入小球一侧的所有腰段背根神经节均有明显标记。两侧的外侧运动柱、腹根以及背根均未标记。这些观察结果表明，在鸡胚中，在感觉细胞对神经生长因子敏感的时期，存在神经生长因子从靶区域到背根神经节的选择性摄取和逆向运输。这些观察结果补充了其他实验室的报告，在这些报告中已证明神经生长因子在新生和成年啮齿动物中存在选择性逆向运输。
