Behzadi Elham, Mahmoodzadeh Hosseini Hamideh, Imani Fooladi Abbas Ali
Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Microb Pathog. 2017 Sep;110:1-6. doi: 10.1016/j.micpath.2017.06.016. Epub 2017 Jun 17.
Bacterial extracellular vesicles (EVs) have come forth into notice as possible important agent to mediate host-pathogen interactions. In this scientific research, the authors have tried to find out the effect of EVs derived from Lactobacillus rhamnosus GG (LDEVs) on the apoptosis induction in HepG2 cell line. The EVs were purified from the conditioned medium of Lactobacillus rhamnosus GG using ultrafiltration and confirmed by transmission electron microscopy (TEM). The HepG2 cells were treated with different concentrations of purified LDEVs and the cytotoxicity and their effects on the expression of bcl-2 and bax genes were assessed by the MTT assay and semi-quantitative RT-PCR, respectively. The MTT assay showed that only 100 μg/ml of LDEVs had a significant cytotoxic effect on cancer cells (p < 0.05). The apoptotic index (bax/bcl2 expression ratio) was significantly increased after treating with 50 and 100 μg/ml LDEVs (p < 0.05). Increased bax/bcl-2 ratio was led to cancer cell death.
细菌细胞外囊泡（EVs）已作为介导宿主-病原体相互作用的潜在重要因子而受到关注。在这项科研中，作者试图探究鼠李糖乳杆菌GG来源的细胞外囊泡（LDEVs）对HepG2细胞系凋亡诱导的影响。使用超滤从鼠李糖乳杆菌GG的条件培养基中纯化细胞外囊泡，并通过透射电子显微镜（TEM）进行确认。用不同浓度的纯化LDEVs处理HepG2细胞，分别通过MTT法和半定量RT-PCR评估细胞毒性及其对bcl-2和bax基因表达的影响。MTT法显示，仅100μg/ml的LDEVs对癌细胞具有显著的细胞毒性作用（p<0.05）。用50和100μg/ml LDEVs处理后，凋亡指数（bax/bcl2表达比值）显著增加（p<0.05）。bax/bcl-2比值增加导致癌细胞死亡。
