Kagirita Atek Atwiine, Baguma Andrew, Owalla Tonny Jimmy, Bazira Joel, Majalija Samuel
Department of Disease Surveillance and Outbreak, Uganda National Health Laboratories Services, Ministry of Health, P.O. Box 7272, Kampala, Uganda.
Department of Microbiology, Faculty of Medicine, Mbarara University of Science and Technology, P.O. Box 1410, Mbarara, Uganda.
Int J Bacteriol. 2017;2017:4604789. doi: 10.1155/2017/4604789. Epub 2017 May 28.
Sporadic outbreaks with varying clinical presentations have been on the rise in various parts of Uganda. The sources of outbreaks and factors underlying the different clinical manifestation are curtailed by paucity of information on genotypes and the associated virulence genes. This study reports molecular diversity of and their genetic virulence profiles among human and animal isolates. Characterization was done using Kauffman-White classification scheme and virulence genes analysis using multiplex PCR. Overall, 52% of the isolates belonged to serogroup D, 16% to serogroup E, 15% to poly F, H-S, and 12% to serogroup B. Serogroups A, C1, and C2 each consisted of only one isolate representing 5%. Virulence genes located on SPI-1 [ and ] and on SPI-2 in addition to and were equally distributed in isolates obtained from all sources. Plasmid encoded virulence gene was found in <5% of isolates from both human epidemic and animal origins whereas it occurred in 80% of clinical isolates. This study reveals that serogroup D is the predominant serogroup in circulation and it is widely shared among animals and humans and calls for joint and coordinated surveillance for one health implementation in Uganda.
乌干达各地偶发性疫情呈上升趋势，临床表现各异。由于缺乏关于基因型和相关毒力基因的信息，疫情源头和不同临床表现背后的因素难以确定。本研究报告了人和动物分离株之间的分子多样性及其遗传毒力特征。使用考夫曼-怀特分类法进行鉴定，并通过多重PCR进行毒力基因分析。总体而言，52%的分离株属于D血清群，16%属于E血清群，15%属于多F、H-S血清群，12%属于B血清群。A、C1和C2血清群各仅包含一个分离株，占5%。位于SPI-1[和]以及SPI-2上的毒力基因，除了和之外，在从所有来源获得的分离株中分布均匀。质粒编码的毒力基因在来自人类疫情和动物源的分离株中均不到5%，而在80%的临床分离株中出现。本研究表明，D血清群是流行中的主要血清群，在动物和人类中广泛存在，并呼吁在乌干达开展联合和协调监测以实施“同一健康”理念。
