Molecular diversity of the gene obtained from human and egg samples.

BACKGROUND AND AIM

Salmonellosis is one of the most common foodborne bacterial diseases in the world. The great majority of infections in humans are foodborne with and Typhimurium accounting for a major part of the problem. The objective of this study was to investigate the presence of gene in strains of isolated from eggs and diarrheal swabs from human cases. In addition, the relationship between A gene nucleotide sequences from different sources (human stool and egg samples) have been studied through phylogenetic tree.

MATERIALS AND METHODS

One hundred and seventy eggs (eggshell and its contents) and 160 stool swabs samples were collected from four poultry farms and medical hospital in Giza Governorate.

RESULTS

The study reported the presence of two strains in eggshell surface with an overall isolation rate of 1.2 and 0% of the egg content. Enteritidis and Typhimurium were isolated from eggshell surface with an incidence of 50% for each strain. Six salmonella strains were isolated from human stool with an incidence of 3.75%; the isolated strains are . Typhimurium, . Enteritidis, Virchow, Haifa, and Kentucky with an incidence of 33.3%, 16.6%, 16.6%, 16.6%, and 16.6%, respectively. Among eight strains, gene was detected with percentage of 50%. The phylogenetic analysis of the sequences gene, from two isolates included in this study and five isolates retrieved from GenBank showed that sequence from human, layer hens, egg, and water in the same clusters.

CONCLUSION

Close relation between drinking contaminated water and layer hens and contaminated water is one such source.