Kadry Mona, Nader Sara Mohamed, Dorgham Sohad M, Kandil Mai M
Department of Zoonoses, Faculty of Veterinary Medicine, Cairo University, Giza 11221, Egypt.
Department of Microbiology and Immunology, National Research Centre, Giza 12622, Egypt.
Vet World. 2019 Jul;12(7):1033-1038. doi: 10.14202/vetworld.2019.1033-1038. Epub 2019 Jul 15.
Salmonellosis is one of the most common foodborne bacterial diseases in the world. The great majority of infections in humans are foodborne with and Typhimurium accounting for a major part of the problem. The objective of this study was to investigate the presence of gene in strains of isolated from eggs and diarrheal swabs from human cases. In addition, the relationship between A gene nucleotide sequences from different sources (human stool and egg samples) have been studied through phylogenetic tree.
One hundred and seventy eggs (eggshell and its contents) and 160 stool swabs samples were collected from four poultry farms and medical hospital in Giza Governorate.
The study reported the presence of two strains in eggshell surface with an overall isolation rate of 1.2 and 0% of the egg content. Enteritidis and Typhimurium were isolated from eggshell surface with an incidence of 50% for each strain. Six salmonella strains were isolated from human stool with an incidence of 3.75%; the isolated strains are . Typhimurium, . Enteritidis, Virchow, Haifa, and Kentucky with an incidence of 33.3%, 16.6%, 16.6%, 16.6%, and 16.6%, respectively. Among eight strains, gene was detected with percentage of 50%. The phylogenetic analysis of the sequences gene, from two isolates included in this study and five isolates retrieved from GenBank showed that sequence from human, layer hens, egg, and water in the same clusters.
Close relation between drinking contaminated water and layer hens and contaminated water is one such source.
沙门氏菌病是世界上最常见的食源性细菌性疾病之一。人类的绝大多数感染是食源性的，鼠伤寒沙门氏菌和肠炎沙门氏菌是问题的主要部分。本研究的目的是调查从鸡蛋和人类病例腹泻拭子中分离的沙门氏菌菌株中A基因的存在情况。此外，还通过系统发育树研究了来自不同来源（人类粪便和鸡蛋样本）的A基因核苷酸序列之间的关系。
从吉萨省的四个家禽养殖场和医院收集了170个鸡蛋（蛋壳及其内容物）和160份粪便拭子样本。
该研究报告在蛋壳表面存在两株沙门氏菌，总体分离率为1.2%，蛋内容物的分离率为0%。肠炎沙门氏菌和鼠伤寒沙门氏菌从蛋壳表面分离出来，每种菌株的发生率为50%。从人类粪便中分离出6株沙门氏菌，发生率为3.75%；分离出的菌株为鼠伤寒沙门氏菌、肠炎沙门氏菌、维尔乔沙门氏菌、海法沙门氏菌和肯塔基沙门氏菌，发生率分别为33.3%、16.6%、16.6%、16.6%和16.6%。在8株沙门氏菌中，检测到A基因的比例为50%。对本研究中的两株分离株和从GenBank检索到的5株分离株的A基因序列进行系统发育分析表明，来自人类、蛋鸡、鸡蛋和水的序列在同一簇中。
饮用受污染的水与蛋鸡之间密切相关，受污染的水就是这样一个来源。
