Ferrarini Marina, Steimberg Nathalie, Boniotti Jennifer, Berenzi Angiola, Belloni Daniela, Mazzoleni Giovanna, Ferrero Elisabetta
Division of Experimental Oncology, San Raffaele Scientific Institute, Via Olgettina 60, 20132, Milan, Italy.
Laboratory of Tissue Engineering, Anatomy and Physiopathology Unit, Department of Clinical and Experimental Sciences, School of Medicine, University of Brescia, Brescia, Italy.
Methods Mol Biol. 2017;1612:177-190. doi: 10.1007/978-1-4939-7021-6_13.
3D-dynamic culture models represent an invaluable tool for a better comprehension of tumor biology and drug response, as they accurately re-create/preserve the complex multicellular organization and the dynamic interactions of the parental microenvironment, which can affect tumor fate and drug sensitivity. Hence, development of models that recapitulate tumor within its embedding microenvironment is an imperative need. This is particularly true for multiple myeloma (MM), which survives almost exclusively in the bone marrow (BM). To meet this need, we have previously exploited and validated an innovative 3D-dynamic culture technology, based on the use of the Rotary Cell Culture System (RCCS ™) bioreactor . Here, we describe, step by step, the procedures we have employed to establish two human MM ex vivo models, i.e., the culture of human BM-derived isolated cells and of MM tissues from patients.
3D动态培养模型是深入理解肿瘤生物学和药物反应的宝贵工具，因为它们能准确地重新创建/保留亲代微环境的复杂多细胞组织和动态相互作用，而这些会影响肿瘤命运和药物敏感性。因此，开发能在其嵌入微环境中重现肿瘤的模型是迫切需求。对于几乎仅在骨髓（BM）中存活的多发性骨髓瘤（MM）而言尤其如此。为满足这一需求，我们此前开发并验证了一种基于旋转细胞培养系统（RCCS™）生物反应器的创新3D动态培养技术。在此，我们逐步描述用于建立两种人MM体外模型的程序，即培养源自人BM的分离细胞和患者的MM组织。
