Sriram Gopu, Bigliardi Paul Lorenz, Bigliardi-Qi Mei
Faculty of Dentistry, National University of Singapore, Singapore, Singapore.
Department of Dermatology, University of Minnesota, Minneapolis, MN, USA.
Methods Mol Biol. 2019;1879:367-383. doi: 10.1007/7651_2018_163.
Atopic dermatitis is a chronic inflammatory skin disease caused by complex multifactorial etiology. In the recent years, there have been significant advances in tissue engineering and the generation of in vitro skin models representative of healthy and diseased states. This chapter describes the methodology for the fabrication of in vitro human skin equivalent (HSE) from human keratinocytes and fibroblasts using a fibrin-based dermal matrix and serum-free culture conditions. Modification of the culture conditions with the supplementation of Th2 cytokines such as interleukin-4 induces the development of atopic dermatitis-like skin model. The chapter also describes the histological and immunohistochemical tools for characterization of the HSE model. The reconstruction of tissue-engineered HSE models that recapitulate the essential features of atopic dermatitis provides powerful tools for deeper understanding of the underlying pathological mechanisms on epidermal level, identification and testing of novel treatment options, and safety and toxicological evaluation in a pathophysiologically relevant system.
特应性皮炎是一种由复杂多因素病因引起的慢性炎症性皮肤病。近年来，组织工程以及代表健康和疾病状态的体外皮肤模型的生成取得了重大进展。本章描述了使用基于纤维蛋白的真皮基质和无血清培养条件，从人角质形成细胞和成纤维细胞制造体外人皮肤等效物（HSE）的方法。用白细胞介素-4等Th2细胞因子补充培养条件可诱导特应性皮炎样皮肤模型的形成。本章还描述了用于表征HSE模型的组织学和免疫组织化学工具。重建能够概括特应性皮炎基本特征的组织工程HSE模型，为在表皮水平深入了解潜在病理机制、识别和测试新的治疗方案以及在病理生理相关系统中进行安全性和毒理学评估提供了有力工具。
