Kim Eun-Kyung, Tang Yujiao, Cha Kwang-Suk, Choi Heeri, Lee Chun Bok, Yoon Jin-Hwan, Kim Sang Bae, Kim Jong-Shik, Kim Jong Moon, Han Weon Cheol, Choi Suck-Jun, Lee Sangmin, Choi Eun-Ju, Kim Sang-Hyun
1 Division of Food Bio Science, College of Biomedical and Health Sciences, Konkuk University , Chungju, Korea.
2 Korea Nokyong Research Center, Konkuk University , Chungju, Korea.
J Med Food. 2015 Aug;18(8):921-8. doi: 10.1089/jmf.2014.3344. Epub 2015 May 19.
The present study aimed to examine the anti-inflammatory effects and potential mechanism of action of Artemisia asiatica Nakai (A. asiatica Nakai) extract in activated murine macrophages. A. asiatica Nakai extract showed dose-dependent suppression of lipopolysaccharide (LPS)-induced nitric oxide, inducible nitric oxide synthase, and cyclooxygenase-2 activity. It also showed dose-dependent inhibition of nuclear factor-κB (NF-κB) translocation from the cytosol to the nucleus and as an inhibitor of NF-κB-alpha phosphorylation. The extract's inhibitory effects were found to be mediated through NF-κB inhibition and phosphorylation of extracellular signal-regulated kinase 1/2 and p38 in LPS-stimulated J774A.1 murine macrophages, suggesting a potential mechanism for the anti-inflammatory activity of A. asiatica Nakai. To our knowledge, this is the first report of the anti-inflammatory effects of A. asiatica Nakai on J774A.1 murine macrophages; these results may help develop functional foods possessing an anti-inflammatory activity.
本研究旨在探讨亚洲龙蒿（Artemisia asiatica Nakai）提取物对活化的小鼠巨噬细胞的抗炎作用及其潜在作用机制。亚洲龙蒿提取物对脂多糖（LPS）诱导的一氧化氮、诱导型一氧化氮合酶和环氧化酶-2活性表现出剂量依赖性抑制作用。它还表现出剂量依赖性抑制核因子-κB（NF-κB）从细胞质向细胞核的转位，并作为NF-κB-α磷酸化的抑制剂。在LPS刺激的J774A.1小鼠巨噬细胞中，发现该提取物的抑制作用是通过抑制NF-κB以及细胞外信号调节激酶1/2和p38的磷酸化介导的，这提示了亚洲龙蒿抗炎活性的潜在机制。据我们所知，这是关于亚洲龙蒿对J774A.1小鼠巨噬细胞抗炎作用的首次报道；这些结果可能有助于开发具有抗炎活性的功能性食品。
