Urban Robotics Laboratory, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Daejeon 34141, Korea.
Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Daejeon 34141, Korea.
Sensors (Basel). 2017 Jun 21;17(6):1459. doi: 10.3390/s17061459.
Simple methods using the striped pattern paper marker and FFT (fast Fourier transformation) have been proposed as alternatives to measuring the optical density for determining the level of bacterial growth. The marker-based method can be easily automated, but due to image-processing-base of the method, the presence of light or the color of the culture broth can disturb the detection process. This paper proposes a modified version of marker-FFT-based growth detection that uses a light emitting diode (LED) array as a marker. Since the marker itself can emit the light, the measurements can be performed even when there is no light source or the bacteria are cultured in a large volume of darkly colored broth. In addition, an LED marker can function as a region of interest (ROI) indicator in the image. We expect that the proposed LED-based marker system will allow more robust growth detection compared to conventional methods.
已经提出了使用条纹图案标记和 FFT（快速傅里叶变换）的简单方法，作为测量光密度以确定细菌生长水平的替代方法。基于标记的方法可以很容易地自动化，但是由于该方法基于图像处理，因此光线的存在或培养肉汤的颜色会干扰检测过程。本文提出了一种基于标记的 FFT 生长检测的改进版本，该方法使用发光二极管（LED）阵列作为标记。由于标记本身可以发光，因此即使没有光源或细菌在大量深色肉汤中培养，也可以进行测量。此外，LED 标记可以在图像中充当感兴趣区域（ROI）的指示器。我们期望与传统方法相比，所提出的基于 LED 的标记系统将允许更稳健的生长检测。
