Pajaro-Castro Nerlis, Caballero-Gallardo Karina, Olivero-Verbel Jesus
Environmental and Computational Chemistry Group, Campus of Zaragocilla, School of Pharmaceutical Sciences, University of Cartagena, Cartagena 130001, Colombia.
Medical and Pharmaceutical Sciences Group, School of Health Sciences, Department of Medicine, University of Sucre, Sincelejo 700003, Colombia.
Int J Environ Res Public Health. 2017 Jun 21;14(6):667. doi: 10.3390/ijerph14060667.
Naphthalene and benzene are widely-used volatile organic compounds. The aim of this research was to examine the toxicological effects of naphthalene and benzene against as an animal model. Adult insects were exposed to these aromatic compounds to assess mortality after 4-48 h of exposure. The lethal concentration 50 (LC) for naphthalene, naphthalin, and benzene were 63.6 µL/L, 20.0 µL/L, and 115.9 µL/L in air, respectively. Real-time polymerase chain reaction (PCR) analysis revealed expression changes in genes related to oxidative stress and metabolism [Glutathione S-Transferase (Gst), and Cytochrome P450 6BQ8 (Cyp6bq8)]; reproduction and metamorphosis [Hormone receptor in 39-like protein (Hr39), Ecdysone receptor: (Ecr), and Chitin synthase 2 (Chs2)]; and neurotransmission [Histamine-gated chloride channel 2 (Hiscl2)] in insects exposed for 4 h to 70.2 µL/L naphthalene. Adults exposed to benzene (80 µL/L; 4 h) overexpressed genes related to neurotransmission [GABA-gated anion channel (Rdl), Hiscl2, and GABA-gated ion channel (Grd)]; reproduction and metamorphosis [Ultraspiracle nuclear receptor (USP), Ecr; and Hr39]; and development (Chs2). The data presented here provides evidence that naphthalene and benzene inhalation are able to induce alterations on reproduction, development, metamorphosis, oxidative stress, metabolism, neurotransmission, and death of the insect.
萘和苯是广泛使用的挥发性有机化合物。本研究的目的是检验萘和苯对[具体动物模型未明确写出]作为动物模型的毒理学影响。将成年昆虫暴露于这些芳香族化合物中，以评估暴露4 - 48小时后的死亡率。萘、萘烷和苯在空气中的半数致死浓度（LC50）分别为63.6 μL/L、20.0 μL/L和115.9 μL/L。实时聚合酶链反应（PCR）分析揭示了在暴露于70.2 μL/L萘4小时的昆虫中，与氧化应激和代谢相关的基因[谷胱甘肽S - 转移酶（Gst）和细胞色素P450 6BQ8（Cyp6bq8）]、与生殖和变态相关的基因[39样蛋白中的激素受体（Hr39）、蜕皮激素受体（Ecr）和几丁质合酶2（Chs2）]以及与神经传递相关的基因[组胺门控氯离子通道2（Hiscl2）]的表达变化。暴露于苯（80 μL/L；4小时）的成虫中，与神经传递相关的基因[γ-氨基丁酸门控阴离子通道（Rdl）、Hiscl2和γ-氨基丁酸门控离子通道（Grd）]、与生殖和变态相关的基因[超气门核受体（USP）、Ecr和Hr39]以及与发育相关的基因（Chs2）过表达。此处呈现的数据提供了证据，表明吸入萘和苯能够诱导昆虫在生殖、发育、变态、氧化应激、代谢、神经传递和死亡方面的改变。
